PM_(2.5)对HK-2细胞氧化损伤和凋亡的影响  

Effects of PM_(2.5) on oxidative damage and apoptosis in HK-2 cells

作　　者：李柏茹 秦双建 关岚[2] 李闰冰 蔡颖[1,3] 蒲桔宁[1,3] 曾明 肖芳[2] 徐新云[1] LI Boru;QIN Shuangjian;GUAN Lan;LI Runbing;CAI Ying;PU Jiening;ZENG Ming;XIAO Fang;XU Xinyun(Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,Guangdong;Xiangya School of Public Health,Central South University,Changsha 410078,Hunan;School of Public Health,University of South China,Hengyang 421001,Hunan,China)

机构地区：[1]深圳市疾病预防控制中心,广东深圳518055 [2]中南大学湘雅公共卫生学院,湖南长沙410078 [3]南华大学公共卫生学院,湖南衡阳421001

出　　处：《癌变．畸变．突变》2021年第5期360-364,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：深圳市科技研发项目(JCYJ20170413101713324,JCYJ20190807102205480)。

摘　　要：目的:探讨深圳和太原市PM_(2.5)对人肾上皮细胞(HK-2)氧化损伤和凋亡的影响。方法:用中流量采样器分别采集太原某大学校园和深圳市疾病预防控制中心楼顶空气,将吸附有PM_(2.5)的滤膜洗脱后制备PM_(2.5)混悬液。设置阴性对照组、50μg/mL深圳PM_(2.5)样品组、50μg/mL太原PM_(2.5)样品组和10μmol/L Cr6+阳性对照组,分别处理HK-2细胞24 h,测定4组细胞氧化损伤指标丙二醛(MDA)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)的变化,并应用流式细胞术检测细胞凋亡水平。结果:与阴性对照组相比,深圳PM_(2.5)样品组、太原PM_(2.5)样品组和阳性对照组中MDA含量分别升高8.16%、34.51%和72.23%;SOD活性分别降低7.49%、19.67%和29.55%;GSH含量分别降低10.43%、16.39%和37.43%。太原PM_(2.5)样品组与阴性对照组的差异均有统计学意义(P<0.05或P<0.01)。GSH-PX活性分别降低42.70%、61.62%和60.98%,细胞凋亡率分别升高197.25%、301.22%和399.08%,上述3组与阴性对照组间的差异均有统计学意义(P<0.05或P<0.01)。结论:深圳和太原市PM_(2.5)均可引起HK-2细胞发生氧化损伤和细胞凋亡率增加,且太原市PM_(2.5)的作用更为明显。OBJECTIVE:To explore effects of PM_(2.5) samples from Shenzhen and Taiyuan on oxidative damage and apoptosis in human kidney 2 cells(HK-2 cells).METHODS:A medium-flow sampler was used to collect air samples at a university campus in Taiyuan and at the top of a building of the Shenzhen Center for Disease Control and Prevention.Membranes from the PM_(2.5)-adsorbed filters were eluted to prepare PM_(2.5) suspensions.HK-2 cultures were divided into several groups:negative control,50μg/mL Shenzhen PM_(2.5),50μg/mL Taiyuan PM_(2.5) and positive control groups.Cells were treated for 24 h and levels of malondialdehyde(MDA),superoxide dismutase(SOD),reduced glutathione(GSH),glutathione peroxidase(GSH-PX)and rates of cell apoptosis were measured.RESULTS:Compared with the negative control group,MDA levels in the Shenzhen,Taiyuan and positive control groups were increased by 8.16%,34.51%and 72.23%,respectively;the SOD levels were reduced by 7.49%,19.67%and 29.55%,respectively;GSH levels were reduced by 10.43%,16.39%and 37.43%,respectively.Among these data,differences between the Taiyuan PM_(2.5) and the positive control groups were statistically significant(P<0.05 or P<0.01).In similar comparisons among the 4 groups,the GSH-PX levels were reduced by 42.70%,61.62%and 60.98%,respectively;the differences between the positive control group,and the Shenzhen and Taiyuan groups were statistically significant(P<0.01).The apoptosis rates were increased by 197.25%,301.22%and 399.08%,respectively;and the differences were statistically significant(P<0.05 or P<0.01).CONCLUSION:Our data indicate that the PM_(2.5) from Shenzhen and Taiyuan caused oxidative damage and increased apoptosis rates in HK-2 cells.In addition,the Taiyuan PM_(2.5) were more toxic than that from Shenzhen.

关 键 词：大气细颗粒物 人肾上皮细胞 氧化损伤 细胞凋亡 

分 类 号：R122.7[医药卫生—环境卫生学]