富血小板血浆和浓缩生长因子对人牙周膜细胞增殖和成骨分化影响的研究  

Effects of platelet-rich plasma and concentrated growth factor on the proliferation and osteogenic differentiation of human periodontal cells

作　　者：刘娟[1] 陈斌[1] 闫福华[1] Liu Juan;Chen Bin;Yan Fuhua(Dept.of Periodontology,Nanjing Stomatological Hospi‐tal,Medical School of Nanjing University,Nanjing 210008,China)

机构地区：[1]南京大学医学院附属口腔医院·南京市口腔医院牙周病科,南京210008

出　　处：《国际口腔医学杂志》2021年第5期520-527,共8页International Journal of Stomatology

基　　金：国家自然科学基金(81771078);南京口腔疾病临床医学研究中心项目(2019060009);江苏省临床医学科技专项(BL2013002)。

摘　　要：目的研究富血小板血浆(PRP)和浓缩生长因子(CGF)对人牙周膜细胞(hPDLCs)增殖及成骨分化的影响。方法分离、培养并鉴定hPDLCs;制备人全血、PRP和CGF,分别进行血小板计数;分别将全血(对照组)、PRP(PRP组)和CGF(CGF组)与hPDLCs共培养,CCK-8法检测细胞增殖能力,细胞划痕实验检测细胞迁移能力;使用1%、5%、10%的PRP和CGF分别对hPDLCs处理24、48、72 h,进行成骨诱导,Western blot法检测成骨相关转录因子Runx2、Osx、Dlx5和Msx2的相对表达量。结果PRP、CGF可促进hPDLCs的增殖和迁移(P<0.05),提高促进成骨相关因子Runx2、Osx、Dlx5的表达并降低抑制成骨因子Msx2的表达(P<0.05)。结论PRP、CGF能促进hPDLCs的增殖和迁移,并促进成骨分化相关转录因子的表达。Objective The study aimed to analyze the effects of platelet-rich plasma(PRP) and concentrated growth factor(CGF) on the proliferation and osteogenic differentiation of human periodontal membrane cells(hPDLCs). Methods The hPDLCs were cultured and isolated from periodontal ligament tissue. PRP and CGF were prepared in accordance with the standard protocols, and platelet count was performed separately. The hPDLCs were cultured to three groups: whole blood(control group), PRP(PRP group), and CGF(CGF group) at various time points. Cell proliferation was detected by the CCK-8 method, and cell migration was detected by the cell scratch assay. The hPDLCs were treated with 1%, 5%, and 10% of PRP and CGF for 24, 48, and 72 h, respectively, for osteogenic induction. Expression levels of osteogenic transcription factors Runx2, Osx, Dlx5, and Msx2 were detected by Western blot. Results Both PRP and CGF could promote the proliferation and migration of the hPDLCs(P<0.05). The expression levels of osteogenic factors Runx2, Osx, and Dlx5 increased significantly;the expression of Msx2, which inhibited osteogenesis, decreased significantly(P<0.05). Conclusion PRP and CGF could promote the proliferation and migration of hPDLCs and induce the expression of osteogenic differentiation-related transcription factors.

关 键 词：富血小板血浆 浓缩生长因子 人牙周膜细胞 细胞增殖 成骨分化 

分 类 号：Q25[生物学—细胞生物学]