不同剂量雷公藤多苷对寻常型银屑病样小鼠血清炎性因子的影响  

Effects of different doses of tripterygium glycosides on serum inflammatory factors in psoriatic vulgaris-like mice

作　　者：宋颖[1] 朱世文[2] 王晓馨[1] SONG Ying;ZHU Shiwen;WANG Xiaoxin(Department of Dermatological,Baoji People’s Hospital,Baoji,Shaanxi 721000,China;Department of Dermatological,Qinghai University Affiliated Hospital,Xining,Qinghai 810001,China)

机构地区：[1]宝鸡市人民医院皮肤科,陕西宝鸡721000 [2]青海大学附属医院皮肤科,青海西宁810001

出　　处：《安徽医药》2021年第10期1934-1938,共5页Anhui Medical and Pharmaceutical Journal

摘　　要：目的观察不同剂量雷公藤多苷对寻常型银屑病样小鼠血清炎性因子白细胞介素-6(IL-6)、白细胞介素-17(IL-17)、白细胞介素-23(IL-23)、肿瘤坏死因子-α(TNF-α)和核转录因子-κB(NF-κB)的影响,并探讨其最佳治疗剂量。方法2019年2—11月,将40只BALB/c雄性小鼠采用随机数字表法分为五组(n=8):对照组、模型组,低、中、高剂量组给药组。小鼠建模后给药组采用不同剂量雷公藤多苷灌胃,浓度为10 mg·kg^(-1)·d^(-1)、20 mg·kg^(-1)·d^(-1)和40 mg·kg^(-1)·d^(-1),持续灌胃14 d。收集小鼠病变皮肤组织,对超氧化物歧化酶(SOD)活性及谷胱甘肽(GSH)和丙二醛(MDA)水平进行测定。酶联免疫吸附测定(ELISA)法检测各组小鼠血清IL-6、IL-17、IL-23和TNF-α水平,Western blotting法检测小鼠血清NF-κB水平。细胞处理后流式细胞术检测各组细胞周期。结果病变皮肤均质化后SOD活性、GSH和MDA水平测定结果显示,模型组小鼠的GSH水平(8.94±0.95)U/mgprot和SOD活性(21.37±3.76)U/mgprot低于对照组(30.93±1.91)U/mgprot、(44.35±4.09)U/mgprot,(P=0.002、0.007),给药组小鼠的GSH水平和SOD活性高于模型组,其中中剂量组最高,为(36.03±1.34)U/mgprot、(42.45±2.43)U/mgprot,P<0.001、P=0.003,低剂量组最低为(26.86±2.03)U/mgprot、(30.39±2.04)U/mgprot,均P<0.05;而MDA测定结果相反,模型组小鼠MDA水平高于对照组,(74.64±2.29)nmol/mgprot比(40.51±2.34)nmol/mgprot,P<0.001,经过药物治疗后降低(P<0.001)。ELISA结果显示,与对照组相比,模型组小鼠血清中IL-6、IL-17、IL-23和TNF-α表达水平升高为(4.93±0.09)pg/mg、(9.05±0.20)pg/mg、(5.23±0.17)pg/mg、(11.51±1.23)pg/mg,均P<0.05;低剂量组小鼠IL-17和TNF-α的表达水平与模型组相比差异无统计学意义(均P>0.05),中剂量组(2.76±0.23)pg/mg、(6.12±0.27)pg/mg、(3.19±0.21)pg/mg、(8.21±0.44)pg/mg和高剂量组(3.72±0.09)pg/mg、(6.02±0.25)pg/mg、(3.37±0.41)pg/mg、(8.44±0.38)pg/mg小鼠血清炎性因子下降,Objective To observe the effects of different doses of tripterygium glycosides on serum inflammatory factors IL-6,IL-17,IL-23,TNF-αand NF-κB in psoriasis vulgaris mice,and to explore the optimal therapeutic dose.Methods From February 2019 to November 2019,40 male BALB/c mice were randomly assigned into 5 groups(n=8)using a random number table method:the blank control group,the model control group,and the low,medium,and high dose groups,respectively.After modelling,the drug-administered group was administered with different doses of tripterygium glycosides at a concentration of 10 mg·kg^(-1)·d^(-1),20 mg·kg^(-1)·d^(-1) and 40 mg·kg^(-1)·d^(-1) for 14 days.Diseased skin tissues of mice were collected,and SOD activity,GSH and MDA levels were measured.The serum levels of IL-6,IL-17,IL-23 and TNF-αin each group of mice were detected by ELISA,and the serum levels of NF-κB were analyzed by Western blotting.After cell treatment,the cell cycle of each group was detected by flow cytometry.Results After homogenization of the diseased skin,the determination result of SOD activity and GSH and MDA levels showed that the GSH[(8.94±0.95)U/mgprot vs.(30.93±1.91)U/mgprot]level and SOD[(21.37±3.76)U/mgprot vs.(44.35±4.09)U/mgprot]activity of mice in model group were significantly lower than those in control group(all P<0.05);GSH and SOD in the drug-administered groups were significantly higher than those in the model group,of which the middle-dose group was the highest[GSH:(36.03±1.34)U/mgprot,SOD:(42.45±2.43)U/mgprot,all P<0.05]and the low dose group was the lowest[(26.86±2.03)U/mgprot,(30.39±2.04)U/mgprot,all P<0.05].However,the results of MDA were reversed.The MDA in the model group[(74.64±2.29)nmol/mgprot]was higher than that in the control group[(40.51±2.34)nmol/mgprot,P<0.001],and it was significantly reduced after drug treatment(P<0.001).The results of ELISA showed that expression levels of IL-6(4.93±0.09)pg/mg,IL-17(9.05±0.20)pg/mg,IL-23(5.23±0.17)pg/mg and TNF-α(11.51±1.23)pg/mg in the model group

关 键 词：雷公藤属 银屑病 超氧化物歧化酶 谷胱甘肽 白细胞介素类 核转录因子-ΚB 小鼠 近交BALB/C 

分 类 号：R285.5[医药卫生—中药学]