基于DNA G-四链体的农兽药残留与重金属离子快速检测研究进展  

Research progress of rapid detection of pesticides and veterinary drugs residues and heavy metal ions based on DNA G-quadruplex

作　　者：孙丽萍 白琳琳[2,3] 干雅婷 陈雪雲 王柳 张宜明 何开雨[2] 徐霞红 SUN Liping;BAI Linlin;GAN Yating;CHEN Xueyun;WANG Liu;ZHANG Yiming;HE Kaiyu;XU Xiahong(College of Agriculture and Food Science,Zhejiang A&F University,Hangzhou 311300,China;Institute of Quality Safety and Nutrition of Agri-Products,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;College of Biological Science and Engineering,Fuzhou University,Fuzhou 350108,China)

机构地区：[1]浙江农林大学农业与食品科学学院,浙江杭州311300 [2]浙江省农业科学院农产品质量安全与营养研究所,浙江杭州310021 [3]福州大学生物科学与工程学院,福建福州350108

出　　处：《浙江农业学报》2021年第9期1770-1778,共9页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金(31701707);浙江省基础公益研究计划(LGF18C200006,LGN19C200021)。

摘　　要：DNA的结构具有多样性,除经典的双螺旋结构外,近年来的研究发现和组装获得了更为多样的DNA结构类型,如三螺旋结构、发夹结构、i-motif结构、G-四链体等非传统结构。这些特殊结构具有一定的功能,例如,G-四链体可与氯高铁血红素结合形成具有辣根过氧化物酶活性的复合物,也可增强某些染料分子的荧光强度。此外,G-四链体的形成与稳定性可控、可设计,是构建化学生物传感的良好材料。以一些典型的研究工作为例,本文对近年来G-四链体在农产品中农兽药残留及重金属离子快速检测中的应用进行了总结,并对其应用前景进行展望。The conformation of DNA are diverse.In addition to the well-known double helix,a number of unconventional structures have been discovered and assembled,such as DNA triple helix,DNA hairpin structure,DNA i-motif,DNA G-quadruplex,and so on.These special DNA structures have some certain functions,for example,DNA G-quadruplexes can bind with hemin and significantly improve the intrinsic peroxidase activity of hemin.Besides this function,they also enhance the fluorescence intensity of some organic dyes.Further more,the formation and stability of G-quadruplexes are programmable and controllable,and they are excellent materials for the construction of chemosensors and biosensors.Taking some typical research works as examples,this paper summarized the application of G-quadruplexes in the rapid detection of pesticide residues,veterinary residues and heavy metals,and the prospective research and application are also discussed.

关 键 词：G-四链体 农药 兽药 重金属离子 比色法 荧光法 快速检测 

分 类 号：S481.8[农业科学—农药学]