硫化氢在大鼠肢体爆炸伤后肾损伤中的作用  

Effects of hydrogen sulfide on kidney injury after extremity explosion injury in rats

作　　者：莫立稳 甯交琳 张凡 赖西南 Mo Li-wen;Ning Jiao-lin;Zhang Fan;Lai Xi-nan(Department of Nephrology,General Hospital of Western Theater Command,Chengdu 610083,China;Institute of field surgery,Army Medical Center of PLA,Chongqing 400042,China)

机构地区：[1]西部战区总医院肾脏病科,成都610083 [2]陆军特色医学中心野战外科研究所,重庆400042

出　　处：《临床肾脏病杂志》2021年第9期755-760,共6页Journal Of Clinical Nephrology

基　　金：全军指令性专项课题基金资助(06Z034)。

摘　　要：目的研究硫化氢(hydrogen sulfide,H 2S)在大鼠肢体爆炸伤后急性肾损伤中的作用,探索H 2S在爆炸伤紧急救治中的价值。方法80 mg二硝基重氮酚纸质点爆源紧贴大鼠左后肢跗关节内侧固定,起爆器引爆致伤。大鼠随机分为致伤组(I组)、硫氢化钠组(NaHS组:10 mg/kg NaHS)、胱硫醚-γ-裂解酶(cystathionine-γ-1yase,CSE)抑制剂组(PAG组,50 mg/kg PAG)和对照组(C组)。致伤6 h后结束实验收集大鼠左肾组织和血标本。测定血尿素氮、血肌酐、血H 2S浓度、左肾组织肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)浓度、丙二醛(malondialdehyde,MDA)浓度和髓过氧化物酶(myeloperoxidase,MPO)活性、超氧化物歧化酶(superoxide dismutase,SOD)活性、酶胱硫醚-γ-裂解酶(cystathionine-γ-1yase,CSE)活性,制作肾组织病理切片观察病理改变。结果与C组相比,I组大鼠肾CSE活性及血浆H 2S浓度显著下降(P<0.01),肾TNF-α、MDA浓度和MPO活性显著升高(P<0.01),而SOD活性则显著下降(P<0.01),给予10 mg/kg NaHS后肾TNF-α、MDA浓度和MPO活性显著降低(P<0.05),SOD活性显著增加(P<0.01);肾病理损害程度也明显减轻;给予PAG后肾TNF-α、MDA浓度和MPO活性较I组显著下降(P<0.05),而SOD活性有所下降,但差异无统计学意义(P>0.05),肾病理损伤也进一步加重。结论大鼠肢体爆炸伤可引起急性肾损伤;肾CSE/H 2S可能通过减轻炎症反应及过氧化反应等发挥肾脏保护作用。Objective To observe the effects of hydrogen sulfide(H 2 S)on kidney injury after extremity explosion injury in rats and search for potential values for explosion injury.Methods Extremity explosion injury was modeled.Healthy male Sprague-Dawley rats were randomly divided into four groups of explosion injury(I,n=8),H 2 S(H 2 S:10 mg/kg NaHS,ip,n=8),PAG(50 mg/kg PAG,ip,n=8)and control(C:n=8).Left kidney and blood samples were harvested.The activity of cystathionine-γ-lyase(CSE)in kidney homogenate and the concentrations of H 2 S,urea nitrogen and creatinine in plasma were detected;the concentrations of tumor necrosis factor-alpha(TNF-α)and the activities of malondialdehyde(MDA),myeloperoxidase(MPO)and superoxide dismutase(SOD)were quantified in kidney homogenate;kidney pathological changes were observed.Results After explosion injury,CSE activity in lung homogenate and H 2 S concentration in plasma decreased markedly(P<0.01);concentrations of TNF-αand MDA and MPO activity in kidney homogenate spiked markedly(P<0.01),SOD activity declined markedly(P<0.01).Severe edema of tubular epithelial cell was noted with an infiltration of inflammatory cells.However in H 2 S group,concentrations of TNF-αand MDA and MPO activity dropped markedly(P<0.05),SOD activity rose markedly(P<0.01)and tubular epithelial cell edema,interstitial renal vascular congestion and inflammatory cell infiltration became greatly relieved.After PAG dosing in PAG group,concentrations of TNF-αand MDA and MPO activity spiked markedly(P<0.05)and SOD activity declined.There was no statistical significance(P>0.05).Conclusion Extremity explosion injury leads to acute kidney injury.And kidney CSE/H 2 S may play an important protective role in acute kidney injury through relieving inflammatory reactions and blunting peroxidation.

关 键 词：爆炸伤 硫化氢 肾损伤 

分 类 号：R692[医药卫生—泌尿科学]