Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Analysis from Active Ingredient of Cyathula Officinalis  

作　　者：MO Ji-hao XIE Han-kun ZHOU Ye-mian Ng Sihan-benjamin LI Shao-xia WANG Lei 

机构地区：[1]Department of Medical Laboratory,Luoyang Orthopedic Hospital of Henan Province,Orthopedic Institute of Henan Province,Luoyang,Henan Province(471002),China [2]Miller School of Medicine,University of Miami(33136),Miami,FL,USA [3]Institute of Technical Biology&Agriculture Engineering,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei(230031),China [4]Eatobe Pte.Limited(573969),Singapore

出　　处：《Chinese Journal of Integrative Medicine》2021年第10期767-773,共7页中国结合医学杂志（英文版）

摘　　要：Objective:To elucidate the active compounds and the molecular mechanism of Cyathula Off/c/na//s as a drug treatment for rheumatoid arthritis(RA).Methods:The target genes of active ingredients from Cyathula Officinalis were obtained from bioinformatics analysis tool for the molecular mechanism of traditional Chinese medicine.The protein-protein interaction between the target genes were analyzed using STRING and Genemania.The transcriptome of RA patients compared to healthy people(GSE121894)were analyzed using R program package Limma.The relative expression of the target genes was obtained from the RNA-seq datasets.The molecular docking analyses were processed based on the molecular model of estrogen receptor 1(ESR1)binding with estradiol(PDB ID:1A52).The binding details were analyzed by SYBYL.Results:Inokosterone,ecdysterone,and cyaterone were the 3 active ingredients from Cyathula Officinalis that bind to target genes.Of all the significantly changed genes from RA patients,ESR1,ADORA1,and ANXA1 were significantly increased in mRNA samples of RA patients.Conclusion:ESR1,the transcription factor that binds inokosterone in the molecular binding analysis,is the target protein of Cyathula Officinalis.

关 键 词：rheumatoid arthritis estrogen receptor 1 inokosterone Cyathula Officinalis Chinese medicine 

分 类 号：R285[医药卫生—中药学]