虎头兰白化茎诱导、再生及人工种子制作  

Induction,Regeneration and Artificial Seed Production of Etiolation Stems of Cymbidium hookerianum

作　　者：付双彬 杨燕萍[1] 周庄[1] Fu Shuangbin;Yang Yanping;Zhou Zhuang(Zhejiang Institute of Subtropical Crops,Wenzhou,325005;Fujian Ornamental Plant Germplasm Resources Innovation&Engineering Application Research Center,Fujian Agriculture and Forestry University,Fuzhou,350002)

机构地区：[1]浙江省亚热带作物研究所,温州325005 [2]福建农林大学,福建省观赏植物种质资源创新与应用工程技术研究中心,福州350002

出　　处：《分子植物育种》2021年第17期5793-5799,共7页Molecular Plant Breeding

基　　金：福建省观赏植物种质资源创新与应用工程技术研究中心开发课题(115/PTJH16005-02);温州农业新品种选育协作组项目(2019ZX004-3)共同资助。

摘　　要：以虎头兰(Cymbidium hookerianum)类原球茎为材料,研究不同激素及激素浓度对类原球茎诱导产生白化茎的影响、后续白化茎的再生以及利用白化茎进行人工种子制作和保存。结果表明,NAA和6-BA各添加0.1 mg/L,类原球茎诱导产生白化茎效率最高,产生的白化茎段在NAA/6-BA为2.0/0.1 mg/L时获得最高的类原球茎诱导率,平均为80.40%,并且类原球茎诱导个数较高,平均为1.36个。产生的白化茎尖在培养基上都能再生成无根植株并且这些无根植株在1/2MS+20.0 g/L蔗糖+7.5 g/L琼脂的培养基上可生根和壮苗。利用白化茎尖包埋产生的人工种子在MS+1.0 mg/L 6-BA培养基上萌发率最高,平均为86.67%。保存6周后,在4℃下保存的人工种子萌发率最高,平均为48.00%。The protocorm-like bodies(PLBs)of Cymbidium hookerianum were used as the explants,the effects of different hormones and hormone concentrations on the induction of etiolation stems,the subsequent regeneration of etiolation stems,and artificial seeds production and conservation using etiolation stems were studied.The results showed that the most efficient etiolation stems production induced by PLBs was obtained by adding 0.1 mg/L NAA and 0.1 mg/L 6-BA.The induced etiolation stems cultivated on the medium containing 2.0/0.1 mg/L NAA/6-BA showed the highest induction rate of PLBs,with an average of 80.40%,and the induction number of PLBs was higher,with an average of 1.36.The induced etiolation shoot tips could regenerate rootless seedlings on the medium,and these seedlings could root and strengthen on the medium of 1/2MS+20.0 g/L sucrose+7.5 g/L agar.The highest germination rate of artificial seeds produced by encapsulating etiolation shoot tips was obtained on the MS medium containing 1.0 mg/L 6-BA,with an average of 86.67%.After storing for 6 weeks,the germination rate of artificial seeds preserved at 4℃was the highest,with an average of 48.00%.

关 键 词：虎头兰(Cymbidium hookerianum) 白化茎 人工种子 

分 类 号：S682.31[农业科学—观赏园艺]