亲水短肽基因NLEAs提高酵母的耐盐能力  

Hydrophilic Short Peptide NLEAs Genes Improving the Salt Tolerance of Yeast

作　　者：武俊杰 丁博 詹瑶 曹雪松 曹高燚 李明 包曙光 谢晓东 Wu Junjie;Ding Bo;Zhan Yao;Cao Xuesong;Cao Gaoyi;Li Ming;Bao Shuguang;Xie Xiaodong(International Joint Research Centre ofMechansim and Genetic Improvement in Stress Tolerance,College of Agricultural&Resource and Environmental Sciences,Tianjin Agricultural University,Tianjin,300384)

机构地区：[1]天津农学院农学与资源环境学院,天津市作物抗逆的机理及遗传改良国际联合研究中心,天津300384

出　　处：《分子植物育种》2021年第17期5886-5890,共5页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31671611,31801446);天津市科技重大专项与工程项目(18ZXBFNC00280)共同资助。

摘　　要：胚胎晚期富集蛋白(late embriogenesis abundant protein,LEA)是植物种子成熟过程中富集的蛋白质,与植物应答逆境胁迫密切相关,具有很强的亲水性。LEA蛋白的增多,有利于降低细胞水势,增强植物耐盐胁迫等逆境的能力。为了进一步提高LEA蛋白的亲水性,提高细胞耐受盐胁迫等渗透胁迫的能力,本研究对LEA蛋白数据库进行了生物信息学分析。结果鉴定出一系列亲水性高的氨基酸序列。对这些序列进行重复及随机组合后,合成了假定的NLEA(Novel LEA)基因。通过筛选转基因酵母菌株,获得了有助于提高酵母耐盐性的NLEA8和NLEA13基因。同时,初步分析了NLEA8和NLEA13基因表达的蛋白的与植物耐盐相关的生物学功能和结构特点。本试验获得了LEA基因,通过酵母耐盐性筛选验证其耐盐能力,对耐盐基因的获得及验证提供了一定的参考。Late embriogenesis abundant protein(LEA)is a protein enriched during the maturation of plant seeds.It is closely related to the response of plants to stress and has a strong hydrophilicity.The increase of LEA protein is conducive to reducing the cell water potential and enhancing the plant's ability to withstand adversity such as salt stress.In order to further improve the hydrophilicity of LEA protein and the ability of cells to withstand osmotic stress such as salt stress,this study conducted a bioinformatics analysis on the LEA protein database.As a result,a series of highly hydrophilic amino acid sequences were identified.After repeating and randomly combining these sequences,a putative NLEA(Novel LEA)gene was synthesized.At the same time,the biological functions and structural characteristics of the proteins expressed by the NLEA8 and NLEA13 genes related to plant salt tolerance were preliminarily analyzed.In this experiment,the LEA gene was obtained,and its salt tolerance was verified by yeast salt tolerance screening,which provided a certain reference for the acquisition and verification of salt tolerance genes.

关 键 词：LEA基因 合成生物学 耐盐性 生物信息学 

分 类 号：Q78[生物学—分子生物学]