一起呼吸道合胞病毒暴发疫情的调查处置及病原学鉴定  

An investigation and pathogen identification of respiratory syncytial virus outbreak in a school

作　　者：颜涛 秦萌[1] 杨霄星[2] 封会茹[1] 尉秀霞[1] 杨洋[2] 余红[1] 李若曦[2] 刘晓玲 董晓根[1] YAN Tao;QIN Meng;YANG Xiao-xing;FENG Hui-ru;WEI Xiu-xia;YANG Yang;YU Hong;LI Ruo-xi;LIU Xiao-ling;DONG Xiao-gen(Department of Microbiological Laboratory,Fengtai District Center for Disease Control and Prevention,Beijing 100071,China;不详)

机构地区：[1]北京市丰台区疾病预防控制中心微生物检验科,北京100071 [2]北京市丰台区疾病预防控制中心传染病与地方病科

出　　处：《现代预防医学》2021年第17期3202-3204,3215,共4页Modern Preventive Medicine

基　　金：国家基金,十三五国家重大专项(2017ZX10103004)。

摘　　要：目的对2019年1月丰台区某校内一起发热暴发疫情进行调查及实验室鉴定。方法使用实时荧光聚合酶链式反应(Real-time fluorescent polymerase chain reaction, RT-PCR)对10份病例咽试子标本进行16种常见呼吸道病毒核酸检测,阳性标本经反转录-巢式聚合酶链式反应(Reverse transcription nested PCR,RT-nested PCR),扩增呼吸道合胞病毒G蛋白高变区部分序列,使用BioEdit及Mega 5.0软件对扩增序列进行同源性分析及进化分析。结果 10份标本中检出7份标本为呼吸道合胞病毒,阳性率为70.0%。序列分析表明检出的呼吸道合胞病毒属于A亚型。结论结合流行病学调查、临床症状及病原学鉴定,初步判断这是一起A亚型呼吸道合胞病毒引起的发热疫情。Objective To investigate and identify the pathogens causing a fever outbreak in a school during January 2019 in Fengtai, Beijing. Methods A total of 10 throat swab samples were collected from the cases to detect respiratory viruses by real-time fluorescent polymerase chain reaction(RT-PCR). The partial sequences of the hypervariable region of respiratory syncytial virus G protein of positive samples were amplified with RT-nested PCR. Alignment and phylogenetic analysis were done by BioEdit and Mega 5.0 software. Results Seven samples were RSV positive(70.0%). The alignment and phylogenetic analysis revealed that the RSV detected belonged to subtype A. Conclusion Combined with epidemiological investigation, clinical symptoms and etiological identification, it is preliminarily determined that this is a fever epidemic caused by respiratory syncytial virus subtype A.

关 键 词：呼吸道合胞病毒 G蛋白 亚型 暴发 

分 类 号：R181.8[医药卫生—流行病学]