三七中皂苷类标准物质的规模化制备分离  

Large-scale preparation and separation of saponins from Panax notoginseng

作　　者：王玥 丁燕[1,2] 朱靖博 WANG Yue;DING Yan;ZHU Jingbo(School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China;Institute of Chemistry and Applications of Plant Resource, Dalian Polytechnic University, Dalian 116034, China)

机构地区：[1]大连工业大学食品学院,辽宁大连116034 [2]大连工业大学植物资源化学与应用研究所,辽宁大连116034

出　　处：《大连工业大学学报》2021年第5期325-328,共4页Journal of Dalian Polytechnic University

基　　金：国家自然科学基金联合基金项目(U1603285);大连市高层次人才项目(2016RQ064).

摘　　要：采用LK1300S小颗粒树脂柱层析结合结晶法,从三七总皂苷提取物中规模化制备分离人参皂苷Rb1。17 kg三七总皂苷提取物吸附于LK1300S小颗粒树脂柱,经70%甲醇水洗脱得到6.3 kg三七皂苷R1和人参皂苷Rg1、Re、Rb1的富集组分。将皂苷富集组分置于甲醇/水体系下进行结晶除杂后质量为5.63 kg,有效提高了组分中目标皂苷纯度。再经LK1300S小颗粒树脂柱进一步分离,得到1.1 kg较纯的人参皂苷Rb1,得率为6.47%,经高效液相色谱检测单体纯度达83.2%。Ginsenoside Rb1 was isolated from total saponins of Panax notoginseng by LK1300S small particle resin column chromatography combined with crystallization.17 kg Panax notoginseng total saponin extract was adsorbed on LK1300S small particle resin column and washed with 70%methanol water then 6.3 kg enriched components of Panax notoginseng saponin R1,ginsenoside Rg1,Re and Rb1 were obtained.The saponin enriched component was crystallized in methanol/water system,and the mass after impurity removal was 5.63 kg,which effectively improved the purity of the target saponin in the component.1.1 kg pure Rb1 was obtained after further separation by LK1300S small particle resin column,with the yield of 6.47%and the monomer purity of 83.2%by HPLC.

关 键 词：三七 人参皂苷RB1 小颗粒树脂柱层析 规模化制备分离 

分 类 号：R284.2[医药卫生—中药学]