The bHLH transcription factor GhPAS1 mediates BR signaling to regulate plant development and architecture in cotton  

作　　者：Huanhuan Wu Zhongying Ren Lei Zheng Mengzhen Guo Jingyu Yang Liyong Hou Ghulam Qanmber Fuguang Li Zuoren Yang 

机构地区：[1]State Key Laboratory of Cotton Biology,Cotton Research Institute of Chinese Academy of Agricultural Sciences,Anyang 455000,Henan,China [2]National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,Hubei,China [3]Zhengzhou Research Base,State Key Laboratory of Cotton Biology,Zhengzhou University,Zhengzhou 450001,Henan,China

出　　处：《The Crop Journal》2021年第5期1049-1059,共11页作物学报（英文版）

基　　金：supported by the Funds for Creative Research Groups of China (31621005);the National Transgenic Major Project of China (2018ZX0800921B)。

摘　　要：Cotton (Gossypium spp.) is the most important natural textile fiber crop in the world.The ideal plant architecture of cotton is suitable for mechanical harvesting and productivity in modern agricultural production.However,cotton genes regulating plant development and architecture have not been fully identified.We identified a basic helix-loop-helix (b HLH) transcription factor,GhPAS1 (PAGODA1 SUPPRESSOR1) in G.hirsutum (Upland cotton).GhPAS1 was located in the nucleus and showed a strong transcription activation effect.Tissue-specific expression patterns showed that GhPAS1 was highly expressed in floral organs,followed by high expression in early stages of ovule development and rapid fiber elongation.GhPAS1 overexpression in Arabidopsis and BRZ (brassinazole,BR biosynthesis inhibitor) treatment indicated that GhPAS1 positively regulates and responds to the BR (brassinosteroid) signaling pathway and promotes cell elongation.GhPAS1 overexpression in Arabidopsis mediated plant development in addition to increasing plant biomass.Virus-induced gene silencing of GhPAS1 indicated that down-regulation of GhPAS1 inhibited cotton growth and development,as plant height,fruit branch length,and boll size of silenced plants were lower than in control plants.Fiber length and seed yield were also lower in silenced plants.We conclude that GhPAS1,a b HLH transcription factor,regulates plant development and architecture in cotton.These findings may help breeders and researchers develop cotton cultivars with desirable agronomic characteristics.

关 键 词：Cotton fiber Basic helix-loop-helix GhPAS1 Plant architecture Virus induced gene silencing 

分 类 号：S562[农业科学—作物学]