Identification and characterization of MeERF genes and their targets in pathogen response by cassava(Manihot esculenta)  

作　　者：Yuhui Hong Yong Xiao Na Song Shousong Zhu Rui Zhao Ke Li Mengting Geng Xiaohui Yu Honggang Wang Wei Xia Yinhua Chen 

机构地区：[1]Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource,College of Tropical Crops,Hainan University,Haikou 570228,Hainan,China [2]Coconut Research Institute,Chinese Academy of Tropical Agricultural Sciences,Wenchang 571339,Hainan,China [3]School of Bioengineering,Dalian University of Technology,Dalian 116024,Liaoning,China

出　　处：《The Crop Journal》2021年第5期1145-1153,共9页作物学报（英文版）

基　　金：supported by the Natural Science Foundation of Hainan Province (2018CXTD330 and 318QN204);Key R&D Program of Hainan Province (ZDYF2019063);China Agriculture Research System (CARS11-hncyh);the National Natural Science Foundation of China (31560497)。

摘　　要：Cassava,Manihot esculenta Crantz (Me),is a major dietary source of calories for over 700 million people in tropical regions.The production of cassava is constantly threatened by cassava bacterial blight (CBB),caused by Xanthomonas axonopodis pv.manihotis (Xam).The gene resources for CBB-resistant breeding of cassava are limited.In model plant species,ethylene response factors play important roles in response to pathogen infection.In this study,cassava ethylene response factors (MeERFs) were identified and characterized as the first step in studying their potential for CBB-resistant breeding of cassava.In the cassava genome 155 MeERFs were identified,of which 23 were induced by Xam infection.The promoter regions of204 genes harbored GCC-box that had the potential to interact with MeERFs.Using 37 transcriptomes derived from Xam infection treatment,four gene co-expression modules for the MeERFs and GCC-box containing genes were constructed.Six MeERFs were associated with two GCC-box containing genes:transcription initiation factor TFIIE subunit beta (MeTFIIE),and histone-lysine N-methyltransferase ASHR1 (MeASHR1).Dual-luciferase reporter assays showed that MeERF10 and MeERF58 positively regulated Me TFIIE;MeERF137 negatively regulated Me TFIIE;MeERF10 and MeERF137 positively regulated Me ASHR1;and MeERF35 negatively regulated Me ASHR1.The four MeERFs may mediate pathogen response by regulating the expression of the two GCC-box containing genes.

关 键 词：CASSAVA Ethylene response factor Expression profile Co-expression analysis PATHOGEN 

分 类 号：S533[农业科学—作物学]