DNA methylation modification in heterosis initiation through analyzing rice hybrid contemporary seeds  

作　　者：Shirong Zhou Meiqing Xing Zhilong Zhao Yincong Gu Yunping Xiao Qiaoquan Liu Hongwei Xue 

机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement,Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China [2]Joint Center for Single Cell Biology,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China [3]National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China [4]Shanghai OEbiotech,Shanghai 201210,China [5]Co-Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province,Key Laboratory of Plant Functional Genomics of the Ministry of Education,Yangzhou University,Yangzhou 225009,Jiangsu,China

出　　处：《The Crop Journal》2021年第5期1179-1190,共12页作物学报（英文版）

基　　金：supported by the Ministry of Science and Technology of China (2012CB944804);the National Transformation Science and Technology Program (2016ZX08001006-009);the National Key Research and Development Program of China (2016YFD0100501, 2016YFD0100902)。

摘　　要：Heterosis is an important biological phenomenon and widely applied in agriculture.Although many studies have been performed by using vegetative organs of F_(1) hybrid plants,how heterosis (or hybrid vigor) is initiated and formed,particularly the underlying molecular mechanism,remain elusive.Hybrid contemporary seeds of rice indica varieties 9311 and PA64 were innovatively used and analysis of DNA methylome of embryo and endosperm at early developing stages revealed the globally decreased DNA methylation.Genes,especially those relate to hormones function and transcriptional regulation present non-additive methylation.Previously identified heterosis-related superior genes are non-additively methylated in early developing hybrid contemporary seeds,suggesting that key genes/loci responsible for heterosis are epigenetically modified even in early developing hybrid seeds and hypomethylation of hybrid seeds after cross-pollination finally result in the long-term transcriptional change of F_(1) hybrid vegetative tissues after germination,demonstrating that altered DNA methylation in hybrid seeds is essential for initiation regulation and maintenance of heterosis exhibiting in F_(1) hybrid plants.Notably,a large number of genes show non-additive methylation in the endosperm of reciprocal hybrids,suggesting that endosperm might also contribute to heterosis.

关 键 词：DNA methylome HETEROSIS RICE Reciprocally hybrid seeds EMBRYO ENDOSPERM 

分 类 号：S511[农业科学—作物学]