肝X受体β基因多态性与2型糖尿病易感性的相关性  

Associations of LXRβ gene polymorphisms and susceptibility to type 2 diabetes

作　　者：罗杰[1] 郭蕾 陈阳希[2] 杨晨[2] 朱小蔚[1] 严妤函 万文辉[2] 张兴虎[2] 刘瑜[2] LUO Jie;GUO Lei;CHEN Yang-xi;YANG Chen;ZHU Xiao-wei;YAN Yu-han;WAN Wen-hui;ZHANG Xing-hu;LIU Yu(Health Office,2.the First Department of Cadre Ward,General Hospital of Eastern Theater Command,PLA,Nanjing 210002,Jiangsu,China)

机构地区：[1]东部战区总医院(原南京军区南京总医院)保健办,南京210002 [2]东部战区总医院(原南京军区南京总医院)干部病房一科,南京210002

出　　处：《东南国防医药》2021年第5期455-459,共5页Military Medical Journal of Southeast China

基　　金：国家自然科学基金(81701890);全军保健专项课题(17BJZ17)。

摘　　要：目的探讨肝X受体β(LXRβ)基因多态性与2型糖尿病(T2DM)易感性的相关性。方法采用病例对照的研究设计,选择2018年1-12月在东部战区总医院体检中心进行健康体检者510例,其中T2DM 128例(T2DM组),对照者382例(对照组)。收集所有研究对象社会人口学资料、既往病史,测量腰臀比,计算体重指数(BMI)。采用改良的连接酶检测反应技术(iMLDR)对LXRβ基因上6个单核苷酸多态性(SNPs)位点进行分型。非条件Logistic回归,校正年龄、性别、吸烟饮酒状态、既往史后,在不同遗传模式下分析各基因型与T2DM易感性的关系;采用协方差分析,校正年龄、性别、吸烟饮酒状态、既往史后,评价其与BMI和腰臀比的相关性。结果 6个SNPs位点在对照组中的频率均符合哈代-温伯格平衡。rs2695121TT基因型[OR (95%CI)=0.48(0.25~0.85),P=0.042]和CT+TT[OR (95%CI)=0.72(0.31~0.98),P=0.038]基因型发生T2DM的风险较CC基因型低,各基因型与BMI和腰臀比无相关性。结论 LXRβ基因多态性与T2DM易感性相关,LXRβ基因变异可能在T2DM发病中起作用。Objective To investigate the relationship between liver X receptor(LXRβ)gene polymorphisms and susceptibility to type 2 diabetes(T2DM).Methods A case-control study design was used to enroll 510 patients who underwent a physical examination in the physical examination centre of our hospital from January 2018 to December 2018,including 128 T2DM patients and 382 controls.Sociodemographic data and medical history were collected,waist-hip ratio(WHR)and body mass index(BMI)were measured and calculated.Six single nucleotide polymorphisms(SNPs)were genotyped using improved ligase detection reaction(iMLDR)genotyping technology.After adjustment for age,gender,smoking and drinking status and past history,the relationship between each genotype and T2DM susceptibility was analyzed under different genetic models by unconditioned logistic regression analysis.Correlation with body mass index(BMI)and WHR was evaluated using covariance analysis.Results The frequencies of six SNPs were in accordance with Hardy-Weinberg equilibrium in the control group.Rs2695121 TT genotype(OR[95%CI]=0.48[0.25-0.85],P=0.042)and CT+TT genotype(OR[95%CI]=0.72[0.31-0.98],P=0.038)had a lower risk of T2DM than CC genotype,and each genotype had no correlation with BMI and WHR.Conclusion LXRβgene mutation may play a role on the pathogenesis of T2DM.

关 键 词：肝X受体β 基因多态性 2型糖尿病 易感性 相关性 

分 类 号：R589[医药卫生—内分泌]