Printed elastic membranes for multimodal pacing and recording of human stem-cell-derived cardiomyocytes  

作　　者：Markos Athanasiadis Dzmitry Afanasenkau Wouter Derks Christoph Tondera Francesca Murganti Volker Busskamp Olaf Bergmann Ivan R.Minev 

机构地区：[1]Biotechnology Center(BIOTEC),Center for Molecular and Cellular Bioengineering(CMCB),Technische Universität Dresden,01307 Dresden,Germany [2]Center for Regenerative Therapies Dresden(CRTD),Center for Molecular and Cellular Bioengineering(CMCB),Technische Universität Dresden,01307 Dresden,Germany [3]Department of Cell and Molecular Biology,Karolinska Institutet,SE-17177 Stockholm,Sweden [4]Present address:Universitätsklinikum Bonn,University of Bonn,Bonn,Germany [5]Present address:Department of Automatic Control and Systems Engineering,University of Sheffield,Mappin Street,Sheffield S13JD,UK [6]These authors contributed equally:Markos Athanasiadis,Dzmitry Afanasenkau,Wouter Derks

出　　处：《npj Flexible Electronics》2020年第1期109-116,共8页npj-柔性电子（英文）

基　　金：We acknowledge funding from Volkswagen Foundation(Freigeist 91690);ERC starting grant(804005‐IntegraBrain)and Center for Advancing Electronics Dresden(cfaed).V.B.was supported by the Volkswagen Foundation(Freigeist A110720);by an ERC starting grant(678071-ProNeurons).

摘　　要：Bioelectronic interfaces employing arrays of sensors and bioactuators are promising tools for the study,repair and engineering of cardiac tissues.They are typically constructed from rigid and brittle materials processed in a cleanroom environment.An outstanding technological challenge is the integration of soft materials enabling a closer match to the mechanical properties of biological cells and tissues.Here we present an algorithm for direct writing of elastic membranes with embedded electrodes,optical waveguides and microfluidics using a commercial 3D printing system and a palette of silicone elastomers.As proof of principle,we demonstrate interfacing of cardiomyocytes derived from human induced pluripotent stem cells(hiPSCs),which are engineered to express Channelrhodopsin-2.We demonstrate electrical recording of cardiomyocyte field potentials and their concomitant modulation by optical and pharmacological stimulation delivered via the membrane.Our work contributes a simple prototyping strategy with potential applications in organ-on-chip or implantable systems that are multi-modal and mechanically soft.

关 键 词：RECORDING MODAL CLOSER 

分 类 号：TB3[一般工业技术—材料科学与工程]