Development and application of molecular markers for TSW(thousand-seed weight)related gene BnaGRF7.C02 in Brassica napus  

作　　者：Jing Zhao Chanyu Jin Rui Geng Yixuan Xue Minqiang Tang Keming Zhu Yulong Li Duoduo Wang Shengyi Liu Xiaoli Tan 

机构地区：[1]School of Life Sciences,Jiangsu University,212013,Jiangsu,China [2]The Oil Crops Research Institute(OCRI)of the Chinese Academy of Agricultural Sciences(CAAS),Wuhan,China

出　　处：《Oil Crop Science》2021年第3期145-150,共6页中国油料作物学报(英文版）

基　　金：the National Key Research and Development Program of China(2016YFD0100305,2016YFD0101900)。

摘　　要：Brassica napus L(rapeseed)is one of the most important oil crops with large cultivated area in China.Seed size and seed weight play crucial roles for yield and harvest.In this study,a type of 15 bp-deletion in BnaGRF7.CO2 coding region was identified through sequence alignment of BnaGRF7.C02 in 42 rapeseed varieties,and associ-ation analysis indicated that the 15 bp-deletion was related to the rapeseed Thousand-Seed Weight(TSW)phenotype.Furthermore,we developed two InDel markers to identify this 15 bp InDel.The tissue-specific expression patterns showed that BnaGRF7.C02 prominently expressed in the late stage of seed development.These findings may assist in InDel markers-based breeding efforts to select higher TWS varieties and improve the crop yield of B.nqpus.

关 键 词：Brassica napus L. BnaGRF7.C02 Thousand-seed weight InDel marker 

分 类 号：S565.4[农业科学—作物学]