镜检和疟原虫抗原快速检测(RDT)胶体金法及巢式PCR法在疟疾诊断中的价值  

Microscopic examination,rapid detection of malaria parasite antigen(RDT)colloidal gold method,nested PCR method in the diagnosis of malaria

作　　者：梁文娇 LIANG Wenjiao(Institute of Microbiology Testing,Chaoyang Municipal Center for Disease Control and Prevention,Liaoning Province,Chaoyang,Liaoning,122000,China)

机构地区：[1]辽宁省朝阳市疾病预防控制中心微生物检测所,辽宁朝阳122000

出　　处：《当代医学》2021年第29期82-84,共3页Contemporary Medicine

摘　　要：目的探究镜检、疟原虫抗原快速检测(RDT)胶体金法、巢式PCR法在疟疾诊断中的价值。方法选取2019年2月至2020年2月本中心收治的疑似疟疾患者50例作为研究对象,所有患者均接受镜检、RDT胶体金法、巢式PCR法,以实验室检测结合临床特征结果为金标准,比较3种方式检测结果。结果实验室检测结合临床特征结果显示,50例疑似疟疾患者中,阳性36例,阴性14例。经镜检法检出疟疾28例,检出率为46.67%;经RDT胶体金法检出疟疾35例,检出率为58.33%;经巢式PCR法检出疟疾30例,检出率为50.00%;RDT胶体金法准确度及灵敏度均高于镜检法、巢式PCR法,差异有统计学意义(P<0.05);3种诊断方法特异度、阳性预测值、阴性预测值比较差异无统计学意义。结论在疟疾临床诊断中,RDT胶体金法具有较高的灵敏度及检出率,且与镜检、巢式PCR法联合诊断,可进一步提高检出率,为临床治疗提供依据。Objective To explore the value of microscopic examination,rapid detection of malaria parasite antigen(RDT)colloidal gold method,nested PCR method in the diagnosis of malaria.Methods 50 patients with suspected malaria received in our center from February 2019 to February 2020 were selected as research subjects.All patients were subjected to microscopy examination,RDT colloidal gold method and nested PCR method,with laboratory testing combined with clinical characteristics as the gold standard,and the test results of the 3 methods were compared.Results The results of laboratory tests combined with clinical characteristics showed that among the 50 suspected malaria patients,36 were positive and 14 were negative.28 cases of malaria were detected by microscopic examination;the detection rate was 46.67%;35 cases of malaria were detected by RDT colloidal gold method,the detection rate was 58.33%;30 cases of malaria were detected by nested PCR method,the detection rate was 50.00%.The detection rate and sensitivity of RDT colloidal gold method were higher than microscopic examination method and nested PCR method,and the difference was statistically significant(P<0.05);there was no significant difference in the specificity,positive predictive value and negative predictive value of the 3 diagnostic methods.Conclusion In the clinical diagnosis of malaria,the RDT colloidal gold method has high sensitivity and detection rate.It can be combined with microscopic examination and nested PCR method to further improve the detection rate and provide an important basis for clinical.

关 键 词：疟疾 镜检 疟原虫抗原快速检测(RDT)胶体金法 巢式PCR法 

分 类 号：R531.3[医药卫生—内科学]