Ethanol extract of Chondracanthus tenellus(Harvey)Hommersand attenuates lipopolysaccharide-induced inflammatory and oxidative response by blocking the NF-κB,MAPKs,and PI3K/Akt signaling pathways  

作　　者：Da Hye Kwon Cheol Park Hyesook Lee Su Hyun Hong Gi-Young Kim Hee-Jae Cha Suhkmann Kim Heui-Soo Kim Hye-Jin Hwang Yung Hyun Choi 

机构地区：[1]Anti-Aging Research Center,Dong-eui University,Busan 47340,Republic of Korea [2]Department of Biochemistry,College of Korean Medicine,Dong-eui University,Busan 47227,Republic of Korea [3]Division of Basic Sciences,College of Liberal Studies,Dong-eui University,Busan 47340,Republic of Korea [4]Department of Marine Life Sciences,School of Marine Biomedical Sciences,Jeju National University,Jeju 63243,Republic of Korea [5]Department of Parasitology and Genetics,College of Medicine,Kosin University,Busan 49104,Republic of Korea [6]Department of Chemistry,College of Natural Sciences,Pusan National University,Busan 46241,Republic of Korea [7]Department of Biological Sciences,College of Natural Sciences,Pusan National University,Busan 46241,Republic of Korea [8]Department of Food and Nutrition,College of Nursing,Healthcare Sciences&Human Ecology,Dong-eui University,Busan 47340,Republic of Korea

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2021年第10期450-459,共10页亚太热带生物医学杂志（英文版）

基　　金：This research was a part of the project titled‘Omics based on fishery disease control technology development and industrialization(20150242)’,funded by the Ministry of Oceans and Fisheries,Republic of Korea。

摘　　要：Objective:To investigate whether the ethanol extract of Chondracanthus tenellus(Harvey)Hommersand,a type of red algae,could exhibit anti-inflammatory potential in lipopolysaccharide(LPS)-stimulated macrophages.Methods:The ethanol extract of Chondracanthus tenellus was applied to 100 ng/mL LPS-stimulated RAW 264.7 cells,and cell viability,phagocytic ability,levels of pro-inflammatory factors,and the production of reactive oxygen species were measured.To identify the underlying mechanism of the ethanol extract of Chondracanthus tenellus,the expression of inflammation-regulated genes was estimated.Results:The ethanol extract of Chondracanthus tenellus had no cytotoxic effect at concentrations below 300μg/mL,and reduced the LPS-induced production of inflammatory mediators including nitric oxide(NO)and prostaglandin E2.Furthermore,the extract markedly suppressed the expression of inducible NO synthase and cyclooxygenase-2,as well as the production of reactive oxygen species.The LPS-induced up-regulation of pro-inflammatory cytokines was attenuated by treatment with the ethanol extract of Chondracanthus tenellus,reducing their extracellular secretion.The Chondracanthus tenellus extract also inhibited LPS-mediated activation of nuclear factor-kappa B(NF-κB).In addition,the phosphorylation of mitogen activated protein kinases(MAPKs)and phosphatidylinositol 3 kinase(PI3K)/Akt was markedly increased by LPS,which was significantly abolished by the Chondracanthus tenellus extract.Conclusions:Our findings indicate that the ethanol extract of Chondracanthus tenellus exhibited potential anti-inflammatory and antioxidant effects through downregulating the NF-κB,MAPKs,and PI3K/Akt signaling pathways in LPS stimulated RAW 264.7 macrophages.tenellus extract.Conclusions:Our findings indicate that the ethanol extract of Chondracanthus tenellus exhibited potential anti-inflammatory and antioxidant effects through downregulating the NF-κB,MAPKs,and PI3K/Akt signaling pathways in LPS stimulated RAW 264.7 macrophages.

关 键 词：Chondracanthus tenellus Inflammation ROS NF-ΚB RAW 264.7 cells 

分 类 号：R392[医药卫生—免疫学]