Comparative study on the chloroplast genomes of five Larix species from the Qinghai-Tibet Plateau and the screening of candidate DNA markers  

作　　者：Qiqiang Guo Huie Li Zengqiang Qian Jie Lu Weilie Zheng 

机构地区：[1]Institute for Forest Resources&Environment of Guizhou/College of Forestry,Guizhou University,Guiyang 550025,People’s Republic of China [2]College of Agriculture,Guizhou University,Guiyang 550025,People’s Republic of China [3]College of Life Sciences,Shaanxi Normal University,Xi’an 710062,People’s Republic of China [4]Key Laboratory of Forest Ecology in Tibet Plateau(Tibet Agriculture&Animal Husbandry University),Ministry of Education,Nyingchi 860000,Tibet,People’s Republic of China [5]Institute of Tibet Plateau Ecology,Tibet Agriculture&Animal Husbandry University,Nyingchi 860000,Tibet,People’s Republic of China

出　　处：《Journal of Forestry Research》2021年第5期2219-2226,共8页林业研究（英文版）

基　　金：the National Natural Science Foundation of China(31660215);the Construction Project for First-Class Ecology Discipline in Guizhou(GNYL[2017]007),China;Major Scientifi c and Technological Projects of Guizhou Province([2018]5261),China。

摘　　要：Five Larix species(L.griffithii,L.speciose,L.himalaica,L.kongboensis,and L.potaninii var.australis),have survived on the Qinghai-Tibet Plateau(QTP)under specific climate conditions for decades.The lack of genomic information seriously hinders research on the evolution,conservation and ecology of these Larix resources.In this study,complete chloroplast(cp)genomes of the 5 species were assembled and compared based on next generation sequencing technology combined with polymerase chain reaction validation.The results show that the 5 cp genomes are relatively conservative in size,gene content and arrangement,and border variation.Phylogenetic analysis showed that the species are closely related as well as to seven other species of the same genus.In addition,the 5 cp genomes contained few simple sequence repeats and relatively low nucleotide variability;thus,12 candidate polymorphic cp DNA markers will be helpful for further research on relevant population genetics.These results will provide valuable genetic information for the conservation,evolution and ecology of these species and their relatives.

关 键 词：Larix species Plastid genome MICROSATELLITE Phylogenetic analysis Qinghai-tibet plateau 

分 类 号：S791.22[农业科学—林木遗传育种]