蛴螬多糖的荧光标记研究  

Study on fluorescent labeling of Polysaccharide of Holotrichia diomphalia larvae

作　　者：张宇洁[1] 杜研 张欣蕾 ZHANG Yu-jie;DU Yan;ZHANG Xin-lei(Department of Pharmacy,Xi'an Medical College,Xi'an 710021,China)

机构地区：[1]西安医学院药学院,陕西西安710021

出　　处：《化学工程师》2021年第8期81-84,共4页Chemical Engineer

基　　金：西安医学院青年基金(205031811)。

摘　　要：用荧光物质8-氨基-1,3,6奈三磺酸二钠盐(ANTS)对蛴螬活性多糖进行荧光标记,对反应时间、温度、pH值、ANTS浓度进行了单因素考察,采用UV-Vis、Flu、HPGFC等方法对标记结果进行确证。结果表明,蛴螬多糖荧光标记的最佳条件是pH值为5.5,40℃条件下反应20h时反应率最高,其荧光取代度为1.56%。实现了荧光检测器检测或追踪多糖,为多糖的生物机制研究及药代动力学的研究提供一定思路。The fluorescent substance 8-amino-1,3,6-naisulfonate disodium salt(ANTS)was used to label the active Polysaccharides of Holotrichia diomphalia larvae.The reaction time,temperature,pH and ANTS concentration were investigated by single factor.The labeling results were confirmed by UV-vis,Flu and HPGFC methods.Single-factor studies showed that the optimum fluorescence labeling condition of Polysaccharides was pH=5.5,40℃for 20h,and the fluorescence substitution degree was 1.56%.The detection and tracking of polysaccharides by fluorescence detector is realized,which provides some ideas for the study of biological mechanism and pharmacokinetics of Polysaccharides.

关 键 词：蛴螬多糖 8-氨基-1 3 6奈三磺酸二钠盐(ANTS) 荧光标记 

分 类 号：TQ460[化学工程—制药化工]