单采献血者IL-28b基因多态性与隐匿性HBV感染相关性研究  

Study on the Correlation between IL-28b Gene Polymorphism and Occult HBV Infection in Platelet Donors

作　　者：周娟[1] 竺青[1] 孙桂芝[1] 张红卫[1] 郭成山 贺韦东[1] 陈敏[1] ZHOU Juan;ZHU Qing;SUN Gui-zhi;ZHANG Hong-wei;GUO Cheng-shan;HE Wei-dong;CHEN Min(Department of Mechanical Collection,Shandong Blood Center,Jinan 250014,China;People’s Hospital of Bao’an District,Guangdong Shenzhen 518101,China)

机构地区：[1]山东省血液中心机采科,济南250014 [2]深圳市宝安区人民医院,广东深圳518101

出　　处：《现代检验医学杂志》2021年第5期8-11,37,共5页Journal of Modern Laboratory Medicine

基　　金：国家自然科学基金(NO.81270577)。

摘　　要：目的研究单采献血者白细胞介素-28b(IL-28b)基因多态性与隐匿性乙型肝炎病毒(HBV)感染(OBI)相关性。方法选择2016年1月~2019年12月山东省血液中心成功捐献血小板HbsAg(-)、核酸检测(NAT)无反应的献血者1158例为研究对象,提取献血者血液DNA,采用直接测序法进行IL-28b基因分型,cobas HBV定量核酸检测试剂盒检测血液HBV-DNA载量,Logistic回归分析IL-28b基因rs8099917位点、rs12979860位点多态性与OBI的关系。结果与非OBI比较,OBI献血者IL-28b基因rs8099917位点G等位基因频率、TG+GG基因型频率显著降低,T等位基因频率、TT基因型频率显著增加(χ^(2)=22.137,22.163,均P<0.01);rs12979860位点T等位基因频率、CT+TT基因型频率显著降低,C等位基因频率、CC基因型频率显著增加(χ^(2)=16.378,19.091,均P<0.01)。OBI献血者rs8099917位点,与TT基因型比较,TG+GG基因型患者HBV-DNA载量显著降低(t=5.257,P<0.01);rs12979860位点,与CC基因型比较,CT+TT基因患者HBV-DNA载量显著降低(t=17.398,P<0.01),以上比较差异均有统计学意义。Logistic回归分析结果显示,rs8099917位点,与TT基因型比较,TG+GG基因型可显著降低OBI发生风险(95%CI:0.288~0.843,P<0.05);rs12979860位点,与CC基因型比较,CT+TT基因型可显著降低OBI发生风险(95%CI:0.207~0.761,P<0.05)。结论单采献血者OBI可能与IL-28b基因rs8099917位点、rs12979860位点多态性有关。Objective To study the correlation between interleukin-28b(IL-28b)gene polymorphism and occult hepatitis B virus(HBV)infection(OBI)in platelet donors.Methods From January 2016 to December 2019,1158 platelet donors from shandong Blood Center who successfully donated platelets with HBsAg(-)and had no response to nucleic acid test(NAT)were selected as the study subjects,DNA was extracted from blood donors,IL-28b was genotyped by direct sequencing,cobas HBV quantitative nucleic acid detection kit was used to detect HBV-DNA load in blood,and Logistic regression was used to analyze the relationships between the polymorphisms of rs8099917,rs12979860 loci on IL-28b gene and OBI.Results Compared with non OBI,the G allele frequency and TG+GG genotype frequency at rs8099917 locus on IL-28b gene in OBI donors were significantly lower,while the T allele frequency and TT genotype frequency were significantly higher(χ^(2)=22.137,22.163,all P<0.01).T allele frequency and CT+TT genotype frequency of rs12979860 were significantly lower,while C allele frequency and CC genotype frequency were significantly higher(χ^(2)=16.378,19.091,all P<0.01).At rs8099917 locus of OBI donors,HBVDNA load of TG+GG genotype was significantly lower than that of TT genotype(t=5.257,P<0.01).At rs12979860 locus,HBVDNA load of CT+TT genotype was significantly lower than that of CC genotype(t=17.398,P<0.01),and the above differences were statistically significant.Logistic regression analysis showed that,at rs8099917 locus,compared with TT genotype,TG+GG genotype could significantly reduced the risk of OBI(95%CI:0.288~0.843,P<0.05).At rs12979860 locus,compared with CC genotype,compared with CC genotype,CT+TT genotype could significantly reduced the risk of OBI(95%CI:0.207~0.761,P<0.05).Conclusion OBI of platelet donors may be related to the polymorphisms of rs8099917 and rs12979860 loci on IL-28b gene.

关 键 词：单采献血者 隐匿性乙型肝炎病毒感染 白细胞介素-28b 基因多态性 

分 类 号：R457.1[医药卫生—治疗学] R512.62[医药卫生—临床医学]