小鼠肌肉损伤修复过程中CTPS及细胞周期相关基因表达变化  

The expression chang of CTPS and cell cycle related genes during the repair of muscle injury in mice

作　　者：梁卫祺 陈雯 叶钊颖 张美晶 苗长久[1] 张伟伟[1,2] 邵淑丽[1,2] LIANG Weiqi;CHEN Wen;YE Zhaoying;ZHANG Meijing;MIAO Changjiu;ZHANG Weiwei;SHAO Shuli(School of Life Sciences,Agriculture and Forestry,Qiqihar University,Qiqihar 161006,China;Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar University,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]齐齐哈尔大学抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江齐齐哈尔161006

出　　处：《高师理科学刊》2021年第9期48-51,共4页Journal of Science of Teachers＇College and University

基　　金：国家青年科学基金项目(31801148);黑龙江省自然科学基金联合引导项目(LH2021C099);黑龙江省教育厅基本业务专项(135209260);黑龙江省省属高等学校基本科研业务费科研项目——植物性食品加工技术特色学科专项(YSTSXK201874);黑龙江省大学生创新创业训练计划项目(202010232058)。

摘　　要：为研究小鼠肌肉损伤修复过程中CTPS基因及细胞周期相关基因CCND1的变化规律,以8周龄雄性昆明小鼠为实验材料,采用注射盐酸布比卡因制备肌肉损伤及修复模型,通过qRT-PCR,Western Blot检测CTPS和CCND1基因的表达变化.结果表明,与对照组相比,注射盐酸布比卡因2~8 h小鼠肌肉组织中CTPS和CCND1基因表达快速增高,且CTPS表达升高早于CCND1;在修复24~72 h过程中,随着时间的延长CTPS和CCND1基因表达量逐渐增高,CTPS基因48 h后增加变缓,CCND1基因在48 h时达到最高.综上所述,在小鼠肌肉修复72 h时间范围内,CTPS和CCND1基因表达升高以满足细胞增殖的需要.研究结果为明确CTPS在肌肉损伤修复中的作用提供实验基础.In order to study the expression changes of CTPS and CCND1 gene during muscle injury repair in mice,8-weeks male Kunming mice were used as experimental material to prepare muscle injury and repair model by injecting bupivacaine hydrochloride.The expression of CTPS and CCND1 gene were detected by qRT-PCR and Western Blot.The results showed that,comparedwith the control group,the expression of CTPS and CCND1 genes in the muscle tissue of mice injected with bupivacaine hydrochloride for 2 to 8 h increased rapidly,and the expression of CTPS increased earlier than that of CCND1,in the repair process from 24 h to 72 h.Among them,the expression of CTPS and CCND1 genes gradually increased with time,the increase of CTPS gene slowed down after 48 h,and the CCND1 gene reached the highest level at 48 h.In summary,within 72 hours of muscle repair in mice,the expression of CTPS and CCND1 genes increased to meet the needs of cell proliferation.The research results provide an experimental basis for clarifying the role of CTPS in muscle injury repair.

关 键 词：CTPS 肌肉损伤修复 细胞周期 

分 类 号：Q25[生物学—细胞生物学]