甜菜BTB蛋白家族的挖掘与生物信息学分析  

Mining and Bioinformatics Analysis of BTB Brotein Family in Sugar Beet

作　　者：刘宇[1,2,3,4,5] 杨巧 王晓丹 赵春雷[1,2,3,4,5] 王希 李彦丽[1,2,3,4,5] 贾海伦 丁广洲[1,2,3,4,5] 陈丽 LIU Yu;YANG Qiao;WANG Xiaodan;ZHAO Chunlei;WANG Xi;LI Yanli;JIA Hailun;DING Guangzhou;CHEN Li(College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080;Sugar Beet Engineering Research Center of Heilongjiang Province,Harbin 150080;National Center for Sugar Crops Improvement,Harbin 150080;Key Labora-tory of Sugar Beet Genetics and Breeding,Heilongjiang Province Common College,Harbin 150080;National Beet Medium-term Gene Bank,Harbin 150080)

机构地区：[1]黑龙江大学现代农业与生态环境学院,哈尔滨150080 [2]黑龙江省甜菜工程技术研究中心,哈尔滨150080 [3]国家糖料改良中心,哈尔滨150080 [4]黑龙江省普通高等学校甜菜遗传育种重点实验室,哈尔滨150080 [5]国家甜菜种质中期库,哈尔滨150080

出　　处：《中国糖料》2021年第4期9-16,共8页Sugar Crops of China

基　　金：黑龙江省经济作物产业技术协同创新体系项目“甜菜育种岗位”(2018711);财政部和农业农村部国家现代农业产业技术体系(糖料)项目“甜菜种质资源收集与评价”(CARS-170102)资助。

摘　　要：BTB(Broad-complex,tramtrack,and bric-à-brac)蛋白在植物生长发育、抗逆性、蛋白质泛素化和降解、细胞骨架组成、离子通道及细胞周期调控等方面发挥着重要作用。本研究从甜菜基因组大数据中获得了49个包含BTB结构域的序列,并利用生物信息学方法对甜菜BTB蛋白家族进行了系统发育、理化性质、基因结构、保守结构域、染色体定位等分析。结果显示:甜菜BTB家族编码的氨基酸大部分都属于酸性氨基酸,氨基酸数目为139~1120个,翻译的蛋白大部分为亲水性蛋白,可分为9个亚家族(Ankyrin、Armadillo、MATH、NPH3、BACK、TAZ、TPR、BTB-only、Other),在染色体上呈不均匀分布,主要分布在第5~7号染色体上;亚细胞定位的结果发现36个BTB蛋白都位于细胞核,其余的位于细胞膜和叶绿体上;49个BTB蛋白的磷酸化位点数目为3~25个,不同蛋白磷酸化位点数目差异很大,49个BTB蛋白的等电点为4.70~9.51,分子量为15848.66~124344.33 Da;甜菜BTB家族不同亚族的基因结构存在较大差异,不同亚族的内含子数量和长度都不同;预测的二级结构和三级结构结果表明,不同亚家族的二级结构以BTB结构域为主,但不同亚族还包含了不同的结构域;三级结构中不同结构的数量虽存在着差异,但都以α螺旋和β折叠为主。本研究通过对甜菜BTB家族的分析,为阐明甜菜BTB家族基因的功能及其机制奠定基础。BTB(Broad-complex,tramtrack,and bric-à-brac)protein plays an important role in plant growth and development,stress resistance,protein ubiquitination and degradation,cytoskeleton composition,ion channels and cell cycle regulation.In this study,49 sequences containing BTB domains were obtained from the data of sugar beet genome,and the physical and chemical properties,gene structure,conserved domains,phylogeny,and chromosomal location of the beet BTB protein family were analyzed using bioinformatics methods.The results showed that most of the amino acids encoded by the BTB family of sugar beet belonged to acidic amino acids,the number of amino acids was 139-1120,and most of the translated proteins were hydrophilic proteins,which could be divided into 9 subfamilies(Ankyrin,Armadillo,MATH,NPH3,BACK,TAZ,TPR,BTB-only and Other).They were unevenly distributed on chromosomes,mainly on chromosomes 5-7.Subcellular localization revealed that 36 BTB proteins were located in the nucleus,while others were located in the cell membrane and chloroplast.The number of phosphorylation sites of 49 BTB proteins was 3-25,and the number of phosphorylation sites of different proteins varied greatly.The isoelectric point of 49 BTB proteins was 4.70-9.51,and molecular weight was 15848.66-24344.33 Da.The gene structure of different subgroups of BTB family of beet was quite different,and the number and length of introns of different subgroups were different.The predicted secondary structure and tertiary structure results showed that the secondary structure of different subfamilies was dominated by BTB domain,but different subfamilies also contained different domains.Although there were differences in the number of different structures in the tertiary structure,they were mainly alpha helix and beta fold.Through the analysis of the beet BTB family,this study laid a foundation for elucidating the function and mechanism of the BTB family genes in sugar beet.

关 键 词：甜菜 BTB蛋白家族 生物信息学分 基因 

分 类 号：S566.3[农业科学—作物学]