苗族药血人参中缩合鞣质类化学成分研究  

Condensed tannins from roots of Indigofera stachyodes

作　　者：张云封[1] 朱枝祥 王文萱 张航 刘莉 张仕林 赵云芳[1] 宋月林 郑姣[1] 屠鹏飞[1] 霍会霞 李军[1] ZHANG Yun-feng;ZHU Zhi-xiang;WANG Wen-xuan;ZHANG Hang;LIU Li;ZHANG Shi-lin;ZHAO Yun-fang;SONG Yue-lin;ZHENG Jiao;TU Peng-fei;HUO Hui-xiaLI Jun(Modern Research Center for Traditional Chinese Medicine,School of Chinese Materia Medica,Beijing University of Chinese Mediciney Beijing 100029,China;Guizhou Hanfang Pharmaceutical Co.,Ltd.,Guiyang 550081,China)

机构地区：[1]北京中医药大学中药学院中药现代研究中心,北京100029 [2]贵州汉方药业有限公司,贵州贵阳550081

出　　处：《中国中药杂志》2021年第16期4131-4138,共8页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划“中医药现代化研究”重点专项(2018YFC1706402,2018YFC1706804)。

摘　　要：利用硅胶、ODS、Sephadex LH-20柱色谱和半制备高效液相色谱技术对苗族药血人参(茸毛木蓝Indigofera stachyodes的干燥根)的化学成分进行研究,通过理化性质和NMR、MS等谱学数据共鉴定了11个缩合鞣质类化合物,分别为血人参鞣质A(1)、epicatechin-(2β→O→7,4β→8)-epiafzelechin-(4β→8)-catechin(2)、肉桂鞣质D1(3)、肉桂鞣质B1(4)、epicatechin-(2β→O→7,4β→8)-epiafzelechin-(4α→8)-epicatechin(5)、gambiriin C(6)、原花青素A1(7)、原花青素A2(8)、aesculitannin B(9)、原花青素A4(10)、原花青素B5(11)。其中化合物1为新化合物,命名为血人参鞣质A,化合物2~11为首次从木蓝属植物中分离得到。化合物1、2、4~11对血小板活化具有明显的抑制作用。Eleven condensed tannins were isolated from the roots of Indigofera stachyodes by various column chromatography techniques including silica gel, octadecyl silica(ODS), Sephadex LH-20, and semi-preparative high performance liquid chromatography(HPLC). These compounds were identified on the basis of physicochemical properties, nuclear magnetic resonance(NMR) and mass spectrometry(MS) data as stachyotannin A(1), epicatechin-(2β→O→7,4β→8)-epiafzelechin-(4β→8)-catechin(2), cinnamtannin D1(3), cinnamtannin B1(4), epicatechin-(2β→O→7,4β→8)-epiafzelechin-(4α→8)-epicatechin(5), gambiriin C(6), proanthocyanidin A1(7), proanthocyanidin A2(8), aesculitannin B(9), proanthocyanidin A4(10), and procyanidin B5(11). Compound 1 is a new compound. Compounds 2-11 were isolated from Indigofera for the first time. Furthermore, compounds 1, 2, and 4-11 showed inhibitory effects on thrombin-induced ATP release in platelets.

关 键 词：血人参 缩合鞣质 化学成分 血小板 

分 类 号：R29[医药卫生—民族医学]