直接注射-多级质谱全扫描法快速定性分析盐生肉苁蓉化学成分  

Rapid chemome profiling of Cistanche salsa using DI-MS/MS

作　　者：曹丽波 龚兴成 贾金茹 曹前 屠鹏飞[1] 宋青青[1] 宋月林 CAO Li-bo;GONG Xing-cheng;JIA Jin-ru;CAO Qian;TU Peng-fei;SONG Qing-qing;SONG Yue-lin(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学中药学院,北京100029

出　　处：《中国中药杂志》2021年第16期4150-4156,共7页China Journal of Chinese Materia Medica

基　　金：国家重点研发计划项目(2018YFC1707300,2017YFC702400);国家自然科学基金项目(82003911,81773875,81530097);中国科协青年人才托举工程项目(2017QNRC001);北京中医药大学青年科学家培育计划项目(BUCM-2019-QNKXJB006)。

摘　　要：该文利用直接注射-多级质谱全扫描(DI-MS/MSALL)技术快速定性分析盐生肉苁蓉的化学成分组成。蠕动泵以10μL·min^(-1)的流速将盐生肉苁蓉提取溶液直接注入Q-TOF质谱,以负离子多级质谱全扫描模式采集图谱,MS1扫描范围为m/z 50~1 200,以m/z 1质谱窗口采集m/z 50~1 200的MS2图谱,共计1 150张MS2图谱。通过归属碎片离子、总结质谱裂解规律,并结合文献和数据库信息等,对主要化学成分进行鉴定,从盐生肉苁蓉提取物中初步鉴定了31个化合物,包括9个苯乙醇苷类化合物、2个环烯醚萜类、4个糖类、9个有机酸类及7个其他类化学成分。通过DI-MS/MSALL技术实现了对盐生肉苁蓉化学成分组成的快速解析,发现其主要化学成分类型与管花肉苁蓉和荒漠肉苁蓉基本类似。该研究为中药化学成分组成的快速定性分析提供了可靠的方法,也为盐生肉苁蓉作为中药肉苁蓉的替代品使用提供了科学证据。The current study aims to rapidly and comprehensively profile the chemical composition of Cistanche salsa using direct infusion coupled with MS/MSALL(DI-MS/MSALL). The C. salsa extract was directly imported into electrospray ionization(ESI) source of quadrupole time-of-flight(Q-TOF) mass spectrometer with an infusion pump at a flow rate of 10 μL·min^(-1). Acquisition program was applied under negative ionization polarity to collect one MS1 spectrum(m/z 50-1 200), followed by 1 150 MS2 spectra with precursor isolation window(m/z 1) amongst mass range m/z 50-1 200. After each MS2 spectrum was matched to its precursor ion, putative identification was conducted through matching mass spectral data with literature and database. A total of 31 components were identified from C. salsa, including 9 phenylethanoid glycosides, 2 iridoids, 4 saccharides, 9 organic acids, and 7 other compounds, similar to those from C. tubulosa and C. deserticola. In conclusion, DI-MS/MSALL, a facile and reliable analytical tool, can be employed for qualitative analysis of chemical constituents in C. salsa. The research offers a promising strategy to achieve rapid chemome profiling of herbal medicine and provides an alternative source of Cistanches Herba.

关 键 词：直接注射-多级质谱全扫描 盐生肉苁蓉 化学成分 

分 类 号：R284.1[医药卫生—中药学]