清热消炎宁胶囊降低HSV-1易感性的清肝泻火作用及机制研究  

Qingre Xiaoyanning Capsules alleviate HSV-1 susceptibility induced by emotional stress

作　　者：欧阳淑桦 王小花 李文[1,2] 罗卓 彭红英 江涛[5] 李怡芳 栗原博 何蓉蓉[1,2,3,4] OUYANG Shu-hua;WANG Xiao-hua;LI Wen;LUO Zhuo;PENG Hong-ying;JIANG Tao;LI Yi-fang;KURIHARA Hiroshi;HE Rong-rong(Guangdong Engineering Research Center of Chinese Medicine&Disease Susceptibility,Jinan University,Guangzhou 510632,China;College of Pharmacy,Jinan University,Guangzhou 510632,China;School of Traditional Chinese Medicine,Jinan University,Guangzhou 510632,China;Integrated Chinese and Western Medicine Postdoctoral Research Station,Jinan University,Guangzhou 510632,China;Guangzhou Baiyunshan Jingxiutang Pharmaceutical Co.,Ltd.,Guangzhou 510130,China)

机构地区：[1]暨南大学广东省疾病易感性及中医药研发工程技术研究中心,广东广州510632 [2]暨南大学药学院,广东广州510632 [3]暨南大学中医学院,广东广州510632 [4]暨南大学中西医结合博士后流动站,广东广州510632 [5]广州白云山敬修堂药业股份有限公司,广东广州510130

出　　处：《中国中药杂志》2021年第16期4208-4213,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(U1801284,81622050);广州市科技计划项目(201903010062)。

摘　　要：该研究采用情志应激诱导1型单纯疱疹病毒(herpes simplex virus type 1,HSV-1)易感模型模拟"肝郁化火"的状态,研究清热消炎宁(Qingre Xiaoyanning,QX)胶囊的清肝泻火作用。将雄性BALB/c小鼠分为正常对照组,HSV-1组,拘束应激+HSV-1组,QX低剂量组(0.658 g·kg^(-1)),QX高剂量组(1.316 g·kg^(-1))和阳性药(阿昔洛韦)组,除正常组和HSV-1组外,其他组小鼠在给药的第3天开始每天拘束6 h,在给药的第9天,除正常组外其他组小鼠滴鼻感染HSV-1,从感染病毒后开始连续观察14 d,记录小鼠体质量变化情况、存活时间、脑炎症状和眼部损伤情况。应用苏木素-伊红染色和免疫组化实验方法检测小鼠脑组织切片病理学变化和HSV-1抗原分布情况;应用空斑法检测小鼠脑组织HSV-1病毒滴度;应用Western blot检测小鼠脑组织中HSV-1相关病毒蛋白ICP27表达水平。实验结果显示,QX能够提高情志应激负荷HSV-1感染小鼠的生存率(P<0.001),降低脑组织中的HSV-1病毒滴度(P<0.01),缓解小鼠脑部炎症(P<0.05)及眼部损伤情况(P<0.05),下调HSV-1相关蛋白ICP27的表达(P<0.05),同时降低脑组织HSV-1抗原分布。上述研究结果表明,QX能够降低情志应激诱导HSV-1易感性,可为HSV-1感染的治疗和预防提供理论依据,推动清肝泻火中药的临床开发及应用。In this study, emotional stress-induced herpes simplex virus type 1(HSV-1) susceptibility model was employed to simu-late the pathological state of " depression-induced liver fire", and the protection effect of Qingre Xiaoyanning(QX) in clearing liver fire was investigated. BALB/c mice were randomly divided into a normal group, a HSV-1 group, a restraint stress + HSV-1 group,low-(0. 658 g·kg^(-1)) and high-dose(1. 316 g·kg^(-1)) QX groups, and an acyclovir group. Except for the normal group and the HSV-1 group, the mice in other groups received daily restraint stress for 6 h from day 3 of medication. On day 9 of medication, mice were anesthetized by isoflurane and infected intranasally with HSV-1. Survival rate, weight change, encephalitis symptoms, and eye injury of mice were recorded for 14 d after virus infection. Hematoxylin-eosin(HE) staining and immunohistochemical staining were used to detect pathological changes and HSV-1 antigen distribution. Plaque assay was performed to detect the titer of HSV-1. The protein ex-pression of ICP27 in the mouse brain was detected by Western blot. The experimental results showed that QX could increase the survival rate of HSV-1-infected mice loaded with emotional stress(P<0. 001), reduce the titer of HSV-1 in the mouse brain(P<0. 01), relieve brain inflammation(P<0. 05) and eye injury(P<0. 05), down-regulate the expression of ICP27 related to HSV-1(P<0. 05), and decrease the distribution of HSV-1 antigen in the mouse brain. The results demonstrated that QX significantly reduced the susceptibility to HSV-1 induced by emotional stress, which is expected to provide a theoretical basis for the treatment and preven-tion of HSV-1 infection and promote the clinical development and application of Chinese medicine effective in clearing liver fire.

关 键 词：情志应激 肝郁化火 1型疱疹病毒 易感性 清热消炎宁胶囊 

分 类 号：R285.5[医药卫生—中药学]