河南省臭椿炭疽病病原鉴定  

Identification of the Pathogen Causing Anthracnose on Ailanthus altissima in Henan Province,China

作　　者：王树和[1] 张佳正 何金鹤 WANG Shu-he;ZHANG Jia-zheng;HE Jin-he(College of Horticulture and Plant Protection,Henan University of Science and Technology,Luoyang 471000,He'nan,China)

机构地区：[1]河南科技大学园艺与植物保护学院,河南洛阳471000

出　　处：《林业科学研究》2021年第5期186-192,共7页Forest Research

基　　金：河南省高等学校重点科研项目(21A210003);河南科技大学博士启动基金(13480059)。

摘　　要：[目的]鉴定引起河南省洛阳市嵩县天池山国家森林公园臭椿炭疽病病原菌,为该病害的防控提供依据。[方法]采用常规组织分离法进行病原分离,利用形态学和多基因系统发育分析相结合的方法对分离菌株鉴定并进行致病性测定。[结果]从病组织中共分离获得11株菌株,所有菌株在PDA培养基上菌落特征表现一致,正面菌落先白色后灰色,背面黑色,分生孢子为圆柱状,两端钝圆,单孢,无色,初步鉴定分离菌株为炭疽菌Colletotrichum spp.。选取代表菌株CH-1和CH-3接种臭椿叶片,有伤条件下接种均能引起臭椿叶片发病,发病叶片症状与田间一致,柯赫氏法则验证成立;经多位点基因(ITS、ACT、TUB2、CHS-1和GAPDH)系统发育分析,发现分离菌株与果生炭疽菌C.fructicola聚集在同一个分支上,支持率达到99%。[结论]本研究首次采用形态学结合多基因系统发育研究,明确发生在河南省洛阳市嵩县天池山国家森林公园的臭椿炭疽病为果生炭疽菌C.fructicola所致。[Objective]The aim of the study is to identify the pathogen causing leaf anthracnose on Ailanthus altissima in the Tianchishan National Forest Park in Songxian County of He'nan Province,China,so as to provide references for the prevention and control of the disease.[Method]Pathogenic fungi were isolated from diseased leaves of A.altissima using tissue isolation methods.The isolates were purified in potato dextrose agar(PDA)by single spore culture.Species identifications for the pathogens causing anthracnose on A.altissima were carried out using morphological characterization,phylogenetic analysis,and pathogenicity assays.[Result]A total of 11 Colletotrichum spp.isolates were recovered from the samples by tissue isolation methods.Colonies were white to gray in color with cottony mycelia and darker underneath on PDA,conidia cylindrical,hyaline,smooth-walled,aseptate.Two representative isolates(CH-1 and CH-3)were selected for pathogenicity tests and phylogenetic analyses.Both the isolates CH-1 and CH-3 were able to infect A.altissima in wounded inoculations.A multi-locus phylogeny was established based on five genomic loci ITS,ACT,TUB2,CHS-1 and GAPDH.The phylogenetic tree showed that the isolates CH-1 and CH-3 from A.altissima clustered into a clade with high confidence(bootstrap value,BP=99%),together with Colletotrichum fructicola.[Conclusion]Based upon morphological characteristics and multi-locus phylogenetic analysis,the isolates CH-1 and CH-3 were identified as C.fructicola.This study represents the first report of C.fructicola on A.altissima in China.

关 键 词：臭椿 炭疽病 果生炭疽菌 病原鉴定 

分 类 号：S432.1[农业科学—植物病理学]