基于多孔金结构的三相界面酶电极的制备及高效电化学酶传感性能  

Fabrication of Triphase Enzyme Electrode Based on Porous Gold Substrate for High-performance Electrochemical Biosensor

作　　者：张嘉懿 丁臻尧 王丹丹[1] 陈礼平 封心建 ZHANG Jiayi;DING Zhenyao;WANG Dandan;CHEN Liping;FENG Xinjian(College of Chemistry,Chemical Engineering and Materials Science;Innovation Center for Chemical Science,Soochow University,Suzhou 215123,China)

机构地区：[1]苏州大学材料与化学化工学部 [2]苏州大学化学科学国际合作创新中心,苏州215123

出　　处：《高等学校化学学报》2021年第10期3167-3174,共8页Chemical Journal of Chinese Universities

基　　金：国家重点研发计划项目(批准号:2019YFA0709200);国家自然科学基金(批准号:21988102,51772198,22002101)资助.

摘　　要：界面微环境是影响酶催化反应及酶传感性能的关键因素.本研究基于三维微纳米结构多孔金基底,通过调控电极表面的亲水和疏水浸润性,制备了具有固-液-气三相界面微环境的氧化酶电极,并研究了界面微环境对酶催化反应动力学的影响规律.基于所制备的三相界面多孔金结构酶电极,反应物氧气能够从气相直接快速地传输到酶催化反应界面,极大地提升了界面氧气浓度及其稳定性,从而大幅度提高了氧化酶活性及酶电极响应的稳定性.以葡萄糖为模型待测物,基于该三相界面酶电极的电化学酶生物传感器拥有宽的线性范围、高的灵敏度、低的检出限以及良好的稳定性.这类独特的三相反应界面设计为高效酶生物传感器的建构以及生物分子的精准检测提供了新思路.The interface microenvironment is the key factor affecting the enzymatic reaction and enzyme bio⁃sensing performance.In this study,we fabricated an oxidase enzyme electrode with an air-liquid-solid triphase interfacial microenvironment by adjusting the surface wettability of three-dimensional(3D)micro/nano-structured porous gold substrate,and studied the effects of interfacial microenvironment on enzymatic reaction kinetics and performance of enzyme electrode.Based on the triphase interface porous gold structured enzyme electrode,the reactant oxygen can be directly and quickly transferred from the gas phase to the enzyme reac⁃tion interface,which greatly increases the interface oxygen concentration and stability,thus enhances the oxi⁃dase activity and the stability of enzyme electrode response.Take glucose as a model analyte,we found that the electrochemical enzyme biosensor has a wide linear range,high sensitivity,low detection limit and good stability.This unique triphase reaction interface design strategy provides a new idea for the construction of high-performance enzyme biosensor and accurate detection of biomolecules.

关 键 词：微结构 润湿性 三相界面 酶催化反应 酶生物传感器 

分 类 号：O643[理学—物理化学]