Refining murine heterotopic heart transplantation:A model to study ischemia and reperfusion injury in donation after circulatory death hearts  

作　　者：Mohammed Quader Renee Cholyway Niluka Wickramaratne Oluwatoyin Akande Martin Mangino Eleonora Mezzaroma Adolfo GMauro Qun Chen Alexander Kantlis Stefano Toldo 

机构地区：[1]Division of Cardiothoracic Surgery,Pauley Heart Center,Virginia Commonwealth University,Richmond,VA,USA [2]School of Pharmacy,Virginia Commonwealth University,Richmond,VA,USA [3]Division of Cardiology,Pauley Heart Center,Virginia Commonwealth University,Richmond,VA,USA

出　　处：《Animal Models and Experimental Medicine》2021年第3期283-296,共14页动物模型与实验医学（英文）

基　　金：Veterans Administration Merit Review,Grant/Award Number:CARA-015-17S;Pauley Heart Center;American Heart Association,Grant/Award Number:16SDG31080002。

摘　　要：Heart transplantation is a lifesaving procedure,which is limited by the availability of donor hearts.Using hearts from donors after circulatory death,which have sustained global ischemia,requires thorough studies on reliable and reproducible models that developing researchers may not have mastered.By combining the most recent lit-erature and our recommendations based on observations and trials and errors,the methods here detail a sound in vivo heterotopic heart transplantation model for rats in which protective interventions on the ischemic heart can be studied,and thus al-lowing the scientific community to advance organ preservation research.Knowledge gathered from reproducible animal models allow for successful translation to clinical studies.

关 键 词：animal models cardiovascular biology cardiovascular disorders REPRODUCIBILITY 

分 类 号：R654.2[医药卫生—外科学] R-332[医药卫生—临床医学]