伏核神经细胞GalR2介导针刀干预神经病理性痛大鼠的镇痛作用  

The GalR2 Mediated Analgesic Effect of Needle Knife in Nucleus Accumbens of Rats with Neuropathic Pain

作　　者：刘亚南 杨双 董玮 李崇阳[3] 徐世莲 LIU Ya-nan;YANG Shuang;DONG Wei;LI Chong-yang;XU Shi-lian(Dept.of Physiology,School of Basic Medicine,Kunming Medical University,Kunming Yunnan 650500;Dept.of Orthopedics,The 3rd Affiliated Hospital of Yunnan Traditional Chinese Medical College,Kunming Yunnan 650041;Dept.of Oncology,The 2nd People’s Hospital of Yunnan Province,Kunming Yunnan 650021,China)

机构地区：[1]昆明医科大学基础医学院生理学系,云南昆明650500 [2]云南中医药大学第三附属医院骨伤科,云南昆明650041 [3]云南省第二人民医院肿瘤科,云南昆明650021

出　　处：《昆明医科大学学报》2021年第9期7-12,共6页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(82160228);昆明医科大学研究生创新基金资助项目(2020S080);云南省应用基础研究计划项目-中医联合基金资助项目(2017FF117)。

摘　　要：目的研究伏核(nucleus accumbens,NAc)神经细胞甘丙肽受体2(galanin receptor 2,GalR2)激活介导针刀干预神经痛的作用机制。方法采用左侧坐骨神经部分结扎(chronic constriction injury,CCI)制作神经痛动物模型,通过测定大鼠对伤害性热刺激诱导的后爪缩爪潜伏期(hind paw withdrawal latency,HWL)和机械刺激诱导的后爪缩爪阈值(hind paw withdrawal threshold,HWT)来检测痛觉阈值。Western blot法检测针刀治疗对CCI大鼠伏核神经细胞GalR2表达的影响,并于CCI大鼠伏核内微量注射甘丙肽受体的阻断剂后,检测其对针刀干预神经痛的痛觉阈值的影响。结果CCI大鼠伏核内注射2 nmoL甘丙肽受体的非特异性阻断剂Galantide减弱了针刀疗法引起的HWL(左侧:P<0.001;右侧:P<0.05)和HWT(左侧:P<0.01;右侧:P<0.05)的延长,但注射1 nmoL的Galantide没有显著性差异(P>0.05)。针刀治疗引起CCI大鼠伏核神经细胞GalR2的表达上调(P<0.01),且注射2 nmoL的GalR2的特异性阻断剂M871后削弱了针刀疗法引起的HWL(左侧:P<0.001;右侧:P<0.001)和HWT(左侧:P<0.001;右侧:P<0.01)。结论CCI大鼠伏核神经细胞GalR2激活可能介导了针刀干预神经痛的镇痛作用。Objective To investigate the mechanism of galanin receptor 2(GalR2)in nucleus accumbens(NAc)mediated needle knife intervention in neuralgia.Methods A mononeuropathy model was duplicated by chronic constriction injury(CCI)of the left sciatic nerve of rat.The hind paw withdraw latency(HWL)to noxious thermal stimulation and the hind paw withdraw threshold(HWT)to noxious mechanical stimulation were tested.Western blot was used to detect the effect of needle knife therapy on the expression of GalR2 in NAc of CCI rats.Finally,the HWL and HWT were tested by intra-NAc injection of galanin receptor blockers after the needle knife therapy in CCI rats.Results The increases of HWL(left:P<0.001;right:P<0.05)and HWT(left:P<0.01;right:P<0.05)induced by the needle knife therapy were reduced by intra-NAc injection of 2 nmol galanin receptors blocker galantide in CCI rats,but not 1 nmol galantide(P>0.05).The expression of GalR2 in NAc of CCI rats was upregulated by the needle knife therapy(P<0.01),and intra-NAc injections of 2 nmol of GalR2 specific blocker M871 weakened the increase of HWL(left:P<0.001;right:P<0.001)and HWT(left:P<0.001;right:P<0.01)induced by the needle knife therapy in CCI rats.Conclusion GalR2 may mediate the analgesic effect of the needle knife therapy in NAc of CCI rats.

关 键 词：GalR2 针刀疗法 神经病理性痛 镇痛作用 

分 类 号：R338.3[医药卫生—人体生理学]