不同大豆品种根瘤菌AFLP分析  

AFLP Analysis of Rhizobium from Different Soybean Varieties

作　　者：姚玉波[1,2] 马春梅[3] 龚振平[3] 柴永山[4] YAO Yubo;MA Chunmei;GONG Zhenping;CHAI Yongshan(Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme,Harbin 150086,China;Institute of Industrial Crops,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;College of Agriculture,Northeast Agricultural Univ.,Harbin 150030,China;Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China)

机构地区：[1]黑龙江省农业科学院博士后科研工作站,哈尔滨150086 [2]黑龙江省农业科学院经济作物研究所,哈尔滨150086 [3]东北农业大学农学院,哈尔滨150030 [4]黑龙江省农业科学院,哈尔滨150086

出　　处：《三峡大学学报（自然科学版）》2021年第5期107-112,共6页Journal of China Three Gorges University：Natural Sciences

基　　金：国家自然科学基金项目(32001479);黑龙江省农业科学院科研项目(2019JJPY004,2020FJZX006);国家重点研发计划项目(2018YFD1000905);黑龙江省应用技术研究与开发计划(GA19B101)。

摘　　要：为研究不同大豆品种不同生育时期的根瘤菌差异,利用AFLP分子标记技术,对12份材料进行分析.结果表明:筛选出的引物组合扩增位点的平均多态性为68.86%.基于遗传相似系数,对12份材料的UPGMA聚类分析共划分为4大类,与大豆品种类型和生育期相关,第一类为黑河41苗期(V_(4))、绥农14苗期(V_(4))、黑河41盛花期(R_(2))、黑农40苗期(V_(4))、秣食豆苗期(V_(4))和秣食豆盛花期(R_(2));第二类为黑农40盛花期(R_(2))、黑河41鼓粒期(R_(6))、黑农40鼓粒期(R_(6))和秣食豆鼓粒期(R_(6));第三类为绥农14盛花期(R_(2));第四类为绥农14鼓粒期(R_(6)).为进一步开展大豆根瘤菌的研究提供参考.In order to study the differences of rhizobium in different growth stages of different soybean varieties,12 materials were analyzed by amplified fragment length polymorphism(AFLP).The results showed that the average polymorphism of the amplified sites of the primer combinations selected was 68.86%.Based on the genetic similarity coefficient UPGMA cluster analysis,12 materials was divided into 4 categories,which were related to soybean type and growth period.The first category were Heihe 41(V_(4)),Suinong 14(V_(4)),Heihe 41(R_(2)),Heinong 40(V_(4)),Moshidou(V_(4))and Moshidou(R_(2));the second category was Heinong 40(R_(2)),Heihe 41(R_(6)),Heinong 40(R_(6))and Moshidou(R_(6));the third category was Suinong 14(R_(2));the fourth category was Suinong 14(R_(6)).Provide reference for further research on soybean rhizobia.

关 键 词：大豆 根瘤菌 AFLP分析 聚类分析 

分 类 号：S565.1[农业科学—作物学]