菠菜NHX家族基因鉴定及其响应盐胁迫的表达分析  

Identification of NHX Family Genes and Its Expression Analysis of Response to Salt Stress in Spinach

作　　者：李茹霞 顾寅钰[1] 李萌[1] 陈传杰[1] 梁晓艳[1] 李俊林 邢延富 张海洋 付娆 Li Ruxia;Gu Yinyu;Li Meng;Chen Chuanjie;Liang Xiaoyan;Li Junlin;Xing Yanfu;Zhang Haiyang;Fu Rao(Shandong Institute of Sericulture,Yantai264002,China;Fushan District Bureau of Agriculture and Rural Affairs,Yantai265500,China)

机构地区：[1]山东省蚕业研究所,山东烟台264002 [2]烟台市福山区农业农村局,山东烟台265500

出　　处：《山东农业科学》2021年第9期1-7,共7页Shandong Agricultural Sciences

基　　金：山东省自然科学基金青年基金项目(ZR2020QC062);山东省农业科学院农业科技创新工程项目(CXGC2021A27);山东省“渤海粮仓”科技示范工程升级版项目(2019BHLC005)。

摘　　要：Na^(+)/H^(+)逆向转运蛋白(NHX)在植物响应盐胁迫和生长发育过程中发挥着重要的调控作用。本研究对菠菜NHX家族基因进行全基因组鉴定和生物信息学分析,并利用qRT-PCR技术检测NHX基因在盐胁迫下的表达。结果显示,从菠菜基因组中共鉴定出6个SpoNHXs基因,系统进化树分析发现其分属3个亚家族——Vac、Endo和PM;这6个SpoNHXs均具有跨膜结构域,与拟南芥NHX家族成员在不同亚家族中的保守基序高度一致,表明其具有相似的生物学功能;这6个SpoNHXs受盐胁迫上调表达,其中SpoNHX1与SpoNHX6显著上调。本研究结果可为后续SpoNHXs基因克隆和耐盐功能分析提供理论支持。Na^(+)/H^(+) antiporter(NHX) family genes play important regulatory roles in plant response to salt stress and growth and development. Thus, in this study, the genome-wide identification of NHX genes in spinach and bioinformatics analysis were carried out, and the expression patterns under salt stress were detected by qRT-PCR. The results showed that six SpoNHXs genes were identified in spinach genome. Phylogenetic analysis showed that they belonged to three subfamilies(Vac, Endo and PM). The six SpoNHXs had transmembrane domains, which were highly consistent with the conserved motifs of Arabidopsis NHX family members in different subfamilies, indicating that they had similar biological functions. The expressions of the six SpoNHXs were up-regulated under salt stress, among which, SpoNHXs and SpoNHX6 were significantly up-regulated. The results of this study could provide references for further cloning of SpoNHXs genes and analysis of salt tolerance function.

关 键 词：菠菜 NHX家族基因 生物信息学分析 盐胁迫 

分 类 号：S636.1[农业科学—蔬菜学]