高效液相色谱法同时检测降糖中成药中的盐酸罗格列酮和盐酸吡格列酮  

HPLC method for examining of rosiglitazone hydrochloride and pioglitazone hydrochloride illegally added in anti-diabetic agents

作　　者：刘舜慧[1] 吴丽萍[1] LIU Shun-hui;WU Li-ping(Department of Pharmacy,Zhangzhou Health Vocational College,Zhangzhou,Fujian 363000)

机构地区：[1]漳州卫生职业学院药学系,福建漳州363000

出　　处：《赣南医学院学报》2021年第9期887-891,共5页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：漳州市科技局重大科技专项项目(zz2018ZD19)。

摘　　要：目的:建立同时检测降糖中成药中非法添加的盐酸罗格列酮和盐酸吡格列酮的高效液相色谱法。方法:采用高效液相色谱法,以Welch Materials XB-C18(250 mm×4.6 mm,5μm)为色谱柱,以甲醇-乙酸铵(0.01 mol∙L^(-1))(62∶38)为流动相,检测波长为246nm,对降糖中成药中非法添加的化学成分进行检测。结果:在选定的色谱条件下,盐酸罗格列酮和盐酸吡格列酮线性范围分别为1.836~91.820μg·mL^(-1)(r=1)、8.2~410μg·mL^(-1)(r=1),平均加样回收率分别为99.76%(RSD=0.51%)、101.01%(RSD=0.37%)。结论:本方法简便、准确、灵敏,可以作为同时检测盐酸罗格列酮和盐酸吡格列酮的方法。Objective:To establish a HPLC method for simultaneously determinating rosiglitazone hydrochloride and pioglitazone hydrochloride illegally added in anti-diabetic agents.Methods:The chromatography was performed using a Welch Materials XB-C18(250 mm×4.6 mm,5μm),with a mobile phase consisting of methanol-ammoniuma acetate(0.01 mol∙L^(-1))(62∶38),detection wavelength at 246 nm,to identify the chemicals illegally added in samples.Results:The range of linearity was 1.836~91.820μg·mL^(-1)for rosiglitazone hydrochloride(r=1),and the average recovery was 99.76%(RSD=0.51%).The range of linearity was 8.2~410μg·mL^(-1)for pioglitazone hydrochloride(r=1),and the average recovery was 101.01%(RSD=0.37%).Conclution:This method was simple and effective with high sensitivity for simultaneously determinating rosiglitazone hydrochloride and pioglitazone hydrochloride.

关 键 词：降糖药 盐酸罗格列酮 盐酸吡格列酮 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]