一株鼠李糖乳杆菌培养条件的优化  

Study on Optimizing Culture Conditions for A Strain of Lactobacillus rhamnosus

作　　者：余萍 张春宇 矫艳平 闵祥博 赵迪 李丽娜 YU Ping;ZHANG Chunyu;JIAO Yanping;MIN Xiangbo;ZHAO Di;LI Lina(High Change(Shenyang)Baby Product Co.Ltd.,Shenyang 110000,China)

机构地区：[1]汉臣氏(沈阳)儿童制品有限公司,沈阳110000

出　　处：《中国乳品工业》2021年第9期28-32,共5页China Dairy Industry

摘　　要：为获得高活菌数的鼠李糖乳杆菌,优化筛选自婴儿粪便的鼠李糖乳杆菌HCS01-003的最适培养条件和筛选优化培养基中最佳促生长因子,研究采用单因素实验设计,分别考察了培养温度、培养基初始pH值、接种量和需氧型对菌体生长的影响;选取了低聚异麦芽糖、低聚半乳糖、水苏糖、维生素B2、维生素B5、维生素B126种物质作为优化培养基中的促生长因子,通过正交实验确定最佳促生长因子。结果表明,鼠李糖乳杆菌HCS01-003最适培养条件为温度37℃,初始pH值为6.8,接种量5%,静置培养;低聚异麦芽糖为鼠李糖乳杆菌最佳促生长因子,最适添加量为低聚异麦芽糖1.5 g/L。通过对培养条件的优化和培养基促生长因子的筛选,为规模化生产奠定基础。To obtain high viable count of Lactobacillus rhamnosus,optimize the optimal culture conditions for Lactobacillus rhamnosus HCS01-003 screened from infant feces,and screening the best growth-promoting factors in optimized medium.The effects of culture temperature,initial pH of medium,inoculum size and aerobic type on the growth of bacteria were investigated by single factor experiment;Isomalto-oligosaccharide,galacto-oligosaccharides,stachyose,vitamin B2,vitamin B5 and vitamin B12 were selected as the growth-promoting factor in the optimized medium,and then best growth-promoting factor was determined by orthogonal test.Results the optimum culture conditions of Lactobacillus rhamnosus HCS01-003 were 37℃,initial pH 6.8,inoculum size 5%and static culture,and the best growth-promoting factor of Lactobacillus rhamnosus was isomalto-oligosaccharide,and the optimal dosage of isomalto-oligosaccharide was 1.5 g/L.The results of this study may provide afoundation for large-scale production。

关 键 词：鼠李糖乳杆菌 培养条件 促生长因子 活菌数 

分 类 号：Q933.31[生物学—微生物学]