Abscisic Acid Connects Phytohormone Signaling with RNA Metabolic Pathways and Promotes an Antiviral Response that Is Evaded by a Self- Controlled RNA Virus  

作　　者：Fabio Pasin Hongying Shan Beatriz García Maren Müller David San León Márta Ludman David H.Fresno Károly Fátyol Sergi Munné-Bosch Guillermo Rodrigo Juan Antonio García 

机构地区：[1]Centro Nacional de Biotecnología(CNB-CSIC),28049 Madrid,Spain [2]Agricultural Biotechnology Research Center,Academia Sinica,11529 Taipei,Taiwan [3]Departamento de Biologı´a Evolutiva,Ecologı´a y Ciencias Ambientales,Facultad de Biologı´a,Universidad de Barcelona,08028 Barcelona,Spain [4]Agricultural Biotechnology Institute,National Agricultural Research and Innovation Centre,2100 Godoll}o,Hungary [5]Institute for Integrative Systems Biology(I2SysBio),CSIC-University of Valencia,46980 Paterna,Spain [6]Present address:College of Horticulture and Gardening,Tianjin Agricultural University,300384 Tianjin,China

出　　处：《Plant Communications》2020年第5期111-128,共18页植物通讯（英文）

基　　金：supported by funds to J.A.G.from the Ministerio de Ciencia e Innovaciòn(Spain),grants BIO2016-80572-R and PID2019-109380RBI00/AEI/10.13039/501100011033(AEI-FEDER);funded by grant K124705 from the National Research Development and Innovation Office(Hungary);S.M.-B.by grant 2017 SGR 980 from the Generalitat de Catalunya(Spain);supported by NIH grant HG006620;the recipient of a post-doctoral fellowship from Academia Sinica(Taiwan).

摘　　要：A complex network of cellular receptors,RNA targeting pathways,and small-molecule signaling provides robust plant immunity and tolerance to viruses.To maximize their fitness,viruses must evolve control mechanisms to balance host immune evasion and plant-damaging effects.The genus Potyvirus comprises plant viruses characterized by RNA genomes that encode large polyproteins led by the P1 protease.A P1 autoinhibitory domain controls polyprotein processing,the release of a downstream functional RNAsilencing suppressor,and viral replication.Here,we show that P1Pro,a plum pox virus clone that lacks the P1 autoinhibitory domain,triggers complex reprogramming of the host transcriptome and high levels of abscisic acid(ABA)accumulation.A meta-analysis highlighted ABA connections with host pathways known to control RNA stability,turnover,maturation,and translation.Transcriptomic changes triggered by P1Pro infection or ABA showed similarities in host RNA abundance and diversity.Genetic and hormone treatment assays showed that ABA promotes plant resistance to potyviral infection.Finally,quantitative mathematical modeling of viral replication in the presence of defense pathways supported self-control of polyprotein processing kinetics as a viral mechanism that attenuates the magnitude of the host antiviral response.Overall,our findings indicate that ABA is an active player in plant antiviral immunity,which is nonetheless evaded by a self-controlled RNA virus.

关 键 词：abscisic acid antiviral immune evasion mathematical modeling viral polyprotein processing POTYVIRUS RNA metabolism 

分 类 号：Q94[生物学—植物学]