A Highly Efficient Agrobacterium-Mediated Method for Transient Gene Expression and Functional Studies in Multiple Plant Species  

作　　者：Youjun Zhang Moxian Chen Beata Siemiatkowska Mitchell Rey Toleco Yue Jing Vivien Strotmann Jianghua Zhang Yvonne Stahl Alisdair R.Fernie 

机构地区：[1]Center of Plant Systems Biology and Biotechnology,4000 Plovdiv,Bulgaria [2]Max-Planck-Institut fur Molekulare Pflanzenphysiologie,Am Muhlenberg 1,14476 Potsdam-Golm,Germany [3]Department of Biology,Hong Kong Baptist University,and State Key Laboratory of Agrobiotechnology,The Chinese University of Hong Kong,Shatin,Hong Kong [4]Institute for Developmental Genetics,Heinrich Heine University,Dusseldorf,Germany

出　　处：《Plant Communications》2020年第5期129-140,共12页植物通讯（英文）

基　　金：supported by funding from the Max Planck Society(A.R.F.and Y.Z.);the European Union’s Horizon 2020 project PlantaSYST SGA-CSA no.739582 under FPA no.664620(A.R.F.and Y.Z.).

摘　　要：Although the use of stable transformation technology has led to great insight into gene function,its application in high-throughput studies remains arduous.Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis,but this technique does not work efficiently in other plant species,including Arabidopsis thaliana.As an efficient high-throughput transient expression system is currently lacking in the model plant species A.thaliana,we developed a method that is characterized by high efficiency,reproducibility,and suitability for transient expression of a variety of functional proteins in A.thaliana and 7 other plant species,including Brassica oleracea,Capsella rubella,Thellungiella salsuginea,Thellungiella halophila,Solanum tuberosum,Capsicum annuum,and N.benthamiana.Efficiency of this method was independently verified in three independent research facilities,pointing to the robustness of this technique.Furthermore,in addition to demonstrating the utility of this technique in a range of species,we also present a case study employing this method to assess protein–protein interactions in the sucrose biosynthesis pathway in Arabidopsis.

关 键 词：transient expression agro-infiltration subcellular localization protein-protein interaction 

分 类 号：Q943.2[生物学—植物学]