A Phelipanche ramosa KAI2 protein perceives strigolactones and isothiocyanates enzymatically  

作　　者：Alexandre de Saint Germain Anse Jacobs Guillaume Brun Jean-Bernard Pouvreau Lukas Braem David Cornu Guillaume Clavé Emmanuelle Baudu Vincent Steinmetz Vincent Servajean Susann Wicke Kris Gevaert Philippe Simier Sofie Goormachtig Philippe Delavault François-Didier Boyer 

机构地区：[1]Institut Jean-Pierre Bourgin,INRAE,AgroParisTech,UniversitéParis-Saclay,78000 Versailles,France [2]Department of Plant Biotechnology and Bioinformatics,Ghent University,9052 Gent,Belgium [3]Center for Plant Systems Biology,VIB,9052 Gent,Belgium [4]Department of Biomolecular Medicine,Ghent University,9000 Ghent,Belgium [5]Center for Medical Biotechnology,VIB,9000 Ghent,Belgium [6]Laboratoire de Biologie et Pathologie Végétales(LBPV),Equipe d’Accueil 1157,Universitéde Nantes,44000 Nantes,France [7]Institute for Biology,Humboldt-Universit€at zu Berlin,10115 Berlin,Germany [8]UniversitéParis-Saclay,CEA,CNRS,Institute for Integrative Biology of the Cell(I2BC),91198,Gif-sur-Yvette,France [9]UniversitéParis-Saclay,CNRS,Institut de Chimie des Substances Naturelles,UPR 2301,91198,Gif-sur-Yvette,France

出　　处：《Plant Communications》2021年第5期132-147,共16页植物通讯（英文）

基　　金：supported by the Institut Jean-Pierre Bourgin’s Plant Observatory technological platforms and has benefited from the facilities and expertise of the I2BC proteomic platform(Proteomic-Gif,SICaPS);supported by Infrastructures en Biologie Santéet Agronomie,Ile de France Region,Plan Cancer,CNRS,and Paris-Sud University;The CHARM3AT Labex program(ANR-11-LABX-39)is also acknowledged for its support;the recipient of an AgreenSkills award from the European Union in the framework of the Marie-Curie FP7 COFUND People Program and a fellowship from Saclay Plant Sciences(ANR-17-EUR-0007);A.J.is indebted to the Research Foundation Flanders for a Structural Basic Research fellowship(project 1S15817N);a travel grant in the framework of a Tournesol fellowship(project VS04418N).

摘　　要：Phelipanche ramosa is an obligate root-parasitic weed that threatens major crops in central Europe.In order to germinate,it must perceive various structurally divergent host-exuded signals,including isothiocyanates(ITCs)and strigolactones(SLs).However,the receptors involved are still uncharacterized.Here,we identify five putative SL receptors in P.ramosa and show that PrKAI2d3 is involved in the stimulation of seed germination.We demonstrate the high plasticity of PrKAI2d3,which allows it to interact with different chemicals,including ITCs.The SL perception mechanism of PrKAI2d3 is similar to that of endogenous SLs in non-parasitic plants.We provide evidence that PrKAI2d3 enzymatic activity confers hypersensitivity to SLs.Additionally,we demonstrate that methylbutenolide-OH binds PrKAI2d3 and stimulates P.ramosa germination with bioactivity comparable to that of ITCs.This study demonstrates that P.ramosa has extended its signal perception system during evolution,a fact that should be considered for the development of specific and efficient biocontrol methods.

关 键 词：a/b-hydrolase receptor seed germination stimulant STRIGOLACTONES ISOTHIOCYANATES Phelipanche ramosa 

分 类 号：Q94[生物学—植物学]