Novel structural annotation and functional expression analysis of GTP_EFTU conserved genes in pepper based on the PacBio sequencing data  

作　　者：Bingqian Tang Lingling Xie Xuefeng Li Huiping Yang Yi Liu Xiang Cheng Chenliang Liang Juan Chen Shudong Zhou Duanhua Wang Jingyuan Zheng Xiongze Dai Xuexiao Zou Feng Liu 

机构地区：[1]Longping Branch,Graduate School of Hunan University,Changsha,Hunan 410125,China [2]Vegetable Institution of Hunan Academy of Agricultural Science,Changsha,Hunan 410125,China [3]College of Landscape and Horticulture,Hunan Agricultural University,Changsha,Hunan 410125,China

出　　处：《Horticultural Plant Journal》2021年第5期443-456,共14页园艺学报（英文版）

基　　金：supported by The General Program of National Natural Science Foundation of China(Grant No.C150202);The National Key Research and Development Programof China(Grant No.2019YFD1000300);The Hunan province Key Research and Development Program of China(Grant No.2019NK2191)。

摘　　要：Genes containing GTP_EFTU domain mainly express elongation factors(EF),Small GTPases,and GTP-binding proteins,which are closely related to protein synthesis,extension and ATP synthesis.In this study,we identified 39 genes containing GTP_EFTU domains from peppers.The evolutionary trees constructed from capsicum,Arabidopsis,rice,and tomato are mainly divided into 7 subfamilies.Using PacBio(Pacific Biosciences)sequencing and assembly data,we extracted these 39 gene sequences,fromwhich 25 genes had alternative splicing.Particularly,the Capana08g000545 had 16 alternative splicing processes.Accordingly,we performed promoter sequence analysis,subcellular location prediction,the expression analysis of different tissues and periods,and also the GO(Gene ontology)analysis of co-expressed genes.Lastly we did the qRTPCR analysis in 5 stages of pepper fruit development.These analyses revealed important structural and functional information for the identified 39 genes that contain GTP_EFTU domains,providing important references for further follow-up experiments to verify the genes function on plants or their unique roles in peppers.

关 键 词：CAPSICUM GTP_EFTU PacBio Phylogenetic analysis Expression analysis GO analysis 

分 类 号：S641.3[农业科学—蔬菜学]