CRISPR ribonucleoprotein-mediated genetic engineering in plants  

作　　者：Yingxiao Zhang Brian Iaffaldano Yiping Qi 

机构地区：[1]Department of Plant Science and Landscape Architecture,University of Maryland,College Park,MD 20742,USA [2]Institute for Bioscience and Biotechnology Research,University of Maryland,Rockville,MD 20850,USA

出　　处：《Plant Communications》2021年第2期38-50,共13页植物通讯（英文）

基　　金：supported by the National Science Foundation Plant Genome Research Program(award nos.IOS-1758745 and IOS-2029889);the U.S.Department of Agriculture Biotechnology Risk Assessment Grant Program(award nos.2018-33522-28789 and 2020-33522-32274);the Emergency Citrus Disease Research and Extension Program(award no.2020-70029-33161);a Foundation for Food and Agriculture Research grant(award no.593603)and Syngenta。

摘　　要：CRISPR-derived biotechnologies have revolutionized the genetic engineering field and have been widely applied in basic plant research and crop improvement.Commonly used Agrobacterium-or particle bombardment-mediated transformation approaches for the delivery of plasmid-encoded CRISPR reagents can result in the integration of exogenous recombinant DNA and potential off-target mutagenesis.Editing efficiency is also highly dependent on the design of the expression cassette and its genomic insertion site.Genetic engineering using CRISPR ribonucleoproteins(RNPs)has become an attractive approach with many advantages:DNA/transgene-free editing,minimal off-target effects,and reduced toxicity due to the rapid degradation of RNPs and the ability to titrate their dosage while maintaining high editing efficiency.Although RNP-mediated genetic engineering has been demonstrated in many plant species,its editing efficiency remains modest,and its application in many species is limited by difficulties in plant regeneration and selection.In this review,we summarize current developments and challenges in RNPmediated genetic engineering of plants and provide future research directions to broaden the use of this technology.

关 键 词：CRISPR RNP genetic engineering genome editing transgene free 

分 类 号：Q943.2[生物学—植物学]