Nucleoporin 160 Regulates Flowering through Anchoring HOS1 for Destabilizing CO in Arabidopsis  

作　　者：Chunying Li Lu Liu Zhi Wei Norman Teo Lisha Shen Hao Yu 

机构地区：[1]Department of Biological Sciences,Faculty of Science,National University of Singapore,Singapore 117543,Singapore [2]Temasek Life Sciences Laboratory,National University of Singapore,Singapore 117604,Singapore

出　　处：《Plant Communications》2020年第2期66-76,共11页植物通讯（英文）

基　　金：This work was supported by the Singapore National Research Foundation Investigatorship Program(NRF-NRFI2016-02);the intramural research support from National University of Singapore and Temasek Life Sciences Laboratory.

摘　　要：Nuclear pore complexes(NPCs),which comprise multiple copies of nucleoporins(Nups),are large protein assemblies embedded in the nuclear envelope connecting the nucleus and cytoplasm.Although it has been known that Nups affect flowering in Arabidopsis,the underlying mechanisms are poorly understood.Here,we show that loss of function of Nucleoporin 160(Nup160)leads to increased abundance of CONSTANS(CO)protein and the resulting upregulation of FLOWERING LOCUS T(FT)specifically in the morning.We demonstrate that Nup160 regulates CO protein stability through affecting NPC localization of an E3-ubiquitin ligase,HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES1(HOS1),which destabilizes CO protein in the morning period.Taken together,these results provide a mechanistic understanding of Nup function in the transition from vegetative to reproductive growth,suggesting that deposition of HOS1 at NPCs by Nup160 is essential for preventing precocious flowering in response to photoperiod in Arabidopsis.

关 键 词：nuclear pore complexes HOS1 CO flowering time 

分 类 号：Q94[生物学—植物学]