PEDV贴壁种毒优化及其在悬浮细胞中的适应性培养  

Optimization of PEDV adherent virus and its adaptive culture in suspension cells

作　　者：李晓梅 潘晓梅 程兰玲 贺笋 黄炯[3] LI Xiaomei;PAN Xiaomei;CHENG Lanling;HE Sun;HUANG Jiong(College of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830009,China;Tecon Biopharmaceutical Co.,Ltd,Urumqi 830000,China;Institute of Veterinary Medicine,Xinjiang Academy of Animal Sciences,Urumqi 830011,China)

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830009 [2]天康生物制药有限公司,乌鲁木齐830000 [3]新疆畜牧科学院兽医研究所,乌鲁木齐830011

出　　处：《黑龙江畜牧兽医》2021年第17期109-113,共5页Heilongjiang Animal Science And veterinary Medicine

摘　　要：为了给猪流行性腹泻病毒(PEDV)XJ-DB2株在悬浮驯化阶段提供稳定的贴壁种毒,验证优化后贴壁种毒在ST悬浮细胞上的感染增殖效果,试验选取3种不同来源的贴壁型Vero细胞,进行连续染毒传代后筛选出传代稳定、病毒效价高的种毒,并测定在ST悬浮细胞上进行驯化传3代后的病毒效价。结果表明:PEDV XJ-DB2株在Zj-Vero细胞上连续驯化21代,病毒效价为1×10^(7.5) TCID_(50)/mL,驯化后的毒株在ST悬浮细胞上连续3代悬浮驯化后,病毒效价为3.16×10^(6) TCID_(50)/mL。说明最适应PEDV XJ-DB2株增殖的贴壁细胞为Zj-Vero细胞,经Zj-Vero细胞驯化后的毒株可以在ST悬浮细胞上增殖培养。In order to provide stable adherent seed strain of Porcine epidemic diarrhea virus(XJ-DB2) in the suspension domestication stage, and to verify the infection and proliferation effect of the optimized adherent seed strain on ST suspension cells, three adherent Vero cells from different sources were selected for continuous inoculation and passage for the screening of the seed strain with stable passage and high virus titer. The virus titer was determined on ST suspension cells after three passages of domestication. The results showed that after 21 successive passages of domestication on Zj-Vero cells, the virus titer of XJ-DB2 strain was 1×10^(7.5) TCID_(50)/mL. After three successive passages of domestication on ST suspension cells with the domesticated strain, the virus titer was 3.16×10^(6) TCID_(50)/mL. The results indicated that Zj-Vero cells were the most suitable adherent cells for the proliferation of XJ-DB2 strain, and the Zj-Vero cell-domesticated strains could be cultured on ST suspension cells.

关 键 词：猪流行性腹泻病毒 悬浮培养 VERO细胞 收获时间 病毒效价 

分 类 号：S852.651[农业科学—基础兽医学]