超临界萃取的海星内脏油成分分析及体外抗肿瘤活性研究  

Composition Analysis and Anti-tumor Effect of Starfish Visceral Oil Extracted by Supercritical Fluid

作　　者：李天娇[1,2,3] 郑莹[1,2,3] 包永睿 王帅[1,2,3] 杨欣欣[1,2,3] 赵国斌[4] 孟宪生 LI Tian-jiao;ZHENG Ying;BAO Yong-rui;WANG Shuai;YANG Xin-xin;ZHAO Guo-bin;MENG Xian-sheng(College of Pharmacy,Liaoning University of Tradition-al Chinese Medicine,Dalian 116600,China;Liaoning Multi-dimensional Analysis of Traditional Chinese Medicine Technical Innovation Center,Liaoning University of Tradition-al Chinese Medicine,Dalian 116600,China;Liaoning Province Modern Traditional Chinese Medicine Research and Engineering Laboratory,Liaoning University of Tradition-al Chinese Medicine,Dalian 116600,China;Dalian Zhuoer Supercritical Technology Development Co.,Ltd.,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁中医药大学辽宁省中药多维分析专业技术创新中心,辽宁大连116600 [3]辽宁中医药大学辽宁省现代中药研究工程实验室,辽宁大连116600 [4]大连卓尔超临界科技发展有限公司,辽宁大连116600

出　　处：《化学试剂》2021年第10期1337-1341,共5页Chemical Reagents

基　　金：辽宁省中药药效物质组学与作用机理研究创新团队项目(LT2017015)。

摘　　要：研究超临界萃取海星内脏油的化学成分组成及其体外抗肿瘤活性,初步探讨其发挥抗肿瘤药效的物质基础。采用超高效液相-飞行时间质谱联用仪(UPLC-QTOF-MS)及气相质谱联用仪(GC-MS)建立超临界萃取海星内脏油的化学成分轮廓谱,将样品分子离子或碎片离子分子质量信息与相关文献或数据库信息进行比对,鉴定化学成分;采用CCK-8法测定超临界萃取海星内脏油对人肝癌HepG2细胞和人结直肠癌SW620细胞的增殖抑制作用。通过UPLC-QTOF-MS共指认出11种非挥发性成分,全部为脂肪酸类成分;GC-MS共检测出38种挥发性成分,其中酯类29种、醇类2种、酸类6种、烷类1种,大部分为脂肪酸类成分,实现了超临界萃取海星内脏油成分的全面表征。海星内脏油对HepG2细胞抑制率的IC_(50)值为0.84 mg/mL,对SW620细胞抑制率的IC_(50)值为0.99 mg/mL,表明其具有抗肝癌及抗肠癌作用,其发挥药效的成分可能为脂肪酸类成分。该研究为海星内脏油作为天然抗肿瘤药物的开发提供理论支撑。To study the chemical composition of starfish visceral oil extracted by supercritical fluid extraction(SFE)and its antitumor activity in vitro,therefore to explore the material basis of its anti-tumor effect.UPLC-QTOF-MS and GC-MS were used to establish the chemical composition profile of starfish visceral oil extracted by supercritical fluid.The molecular weight information of molecular ions or fragment ions of samples was compared with the relevant literatures or databases to identify the chemical composition of starfish visceral oil.CCK-8 method was used to determine the inhibitory effect of starfish visceral oil on the proliferation of HepG2 cells and SW620 cells.A total of 11 components were identified by UPLC-QTOF-MS,and they are all fatty acids.A total of 38 components were detected by GC-MS,including 29 esters,2 alcohols,6 acids and 1 alkane,and most of which are fatty acids.The comprehensive characterization of starfish visceral oil by supercritical fluid extraction was realized.The IC_(50)of starfish visceral oil on HepG2 cells was 0.84 mg/mL,and that on SW620 cells was 0.99 mg/mL,which indicated that starfish visceral oil has anti-hepatoma and anti-colorectal cancer effects.The anti-cancer components may be fatty acids.This study provides theoretical support for the development of starfish visceral oil as a natural anti-tumor drug.

关 键 词：海星内脏油 超临界萃取 成分分析 抗肿瘤活性 超高效液相-飞行时间质谱 气相质谱 

分 类 号：R9[医药卫生—药学]