浊点萃取-高效液相色谱法测定工业废水中4种苯酚类化合物  

Determination of Four Phenols in Industrial Wastewater by Cloud Point Extraction-high Performance Liquid Chromatography

作　　者：刘甜恬 杨晓颖 姜勋 谢美仪 郝晓棠 罗幸玲 王梅 LIU Tian-tian;YANG Xiao-ying;JIANG Xun;XIE Mei-yi;HAO Xiao-tang;LUO Xing-ling;WANG Mei(School of Pub-lic Health,Guangdong Pharmaceutical University,Guangzhou 510310,China)

机构地区：[1]广东药科大学公共卫生学院,广东广州510310

出　　处：《化学试剂》2021年第10期1381-1387,共7页Chemical Reagents

基　　金：广州市科技计划项目(202002030409);广东省教育厅创新强校工程项目(2019GCZX012)。

摘　　要：建立了浊点萃取法与高效液相色谱联用测定4种苯酚类化合物(对苯二酚、间苯二酚、邻苯二酚和苯酚)的分析方法。以磷酸三丁酯为配合剂,Tergitol 15-S-7为萃取剂,4种目标化合物与磷酸三丁酯通过氢键作用力形成了易于被Tergitol 15-S-7萃取的疏水性配合物,解决了苯酚类化合物萃取率低的问题。实验优化了配合剂、非离子表面活性剂和无机盐的种类和用量、溶液pH,以及水浴温度和平衡时间。最优条件下,在0.25~350μg/L浓度范围内,4种目标化合物的浓度与峰面积均呈良好线性关系,其线性相关系数≥0.9995,检出限(LOD,S/N=3)为0.05~0.09μg/L,萃取率为77.4%~87.6%。方法成功用于分离检测工业废水中4种苯酚类化合物,样品加标回收率在90.2%~110%之间。A novel method was established for the determination of 4 phenols compounds(hydroquinone,resorcinol,catechol and phenol)by cloud point extraction and high performance liquid chromatography.The nonionic surfactant Tergitol 15-S-7 was chosen as the extractant,meanwhile,the tributyl phosphate(TBP)was used as a complexant.The 4 analytes were quantitatively extracted in the form of hydrophobic complexes with TBP by Tergitol 15-S-7.Therefore,the problem that the traditional CPE has poor extraction effect on four hydrophilic phenols was solved.The effect of experimental factors such as the type and concentration of complexant,surfactant and inorganic salts,the pH value of solution,equilibrium temperature and time were investigated in detail.Under optimal conditions,the 4 analytes showed good linearity in the concentration range of 0.25~350μg/L,and the linear correlation coefficient were≥0.9995 with the limits of detection(LODs,S/N=3)of 0.05~0.09μg/L.Good extraction efficiencies in the ranges of 77.4%~87.6%were obtained.The proposed method was successfully applied to simultaneous determination of the four phenols compounds in industrial waste water samples with recoveries 90.2%~110%.

关 键 词：浊点萃取 高效液相色谱 苯酚类化合物 工业废水 

分 类 号：X832[环境科学与工程—环境工程]