单分散四臂聚乙二醇衍生的高选择性Hg^(2+)荧光探针的合成和性能研究  

Synthesis and Properties of Highly Selective Hg^(2+)Ions Fluorescence Probe Derived Monodisperse 4-Arm-PEG

作　　者：张安林 彭媛媛 张径 陈芳军 邓泽平 ZHANG An-lin;PENG Yuan-yuan;ZHANG Jing;CHEN Fang-jun;DENG Ze-ping(Postdoctoral Scientific Research Cooperative R&D Center,Hunan Huateng Pharmaceutical Co.,Ltd.,Changsha 410006,China;College of Pharmacy,Changsha Medical University,Changsha 410219,China)

机构地区：[1]湖南华腾制药有限公司博士后流动站协作研发中心,湖南长沙410006 [2]长沙医学院药学院,湖南长沙410219

出　　处：《化学试剂》2021年第10期1419-1427,共9页Chemical Reagents

基　　金：湖南省创新创业科技投资项目(2018GK5002);湖南省创新型省份建设专项社会发展领域重点研发项目(2019SK2211);湖南省创新型省份建设专项高新技术产业科技创新引领计划项目(2020SK2038)。

摘　　要：为了提高荧光探针的潜在应用价值,研究开发了一种基于单分散四臂聚乙二醇的罗丹明B衍生物荧光探针,该探针在水溶液中对Hg^(2+)金属离子具有高选择性。与其他竞争性金属阳离子相比,在有Hg^(2+)存在的情况下,探针在572 nm处表现出显著的荧光增强。探针的荧光信号变化是基于严格的"开-关"机制,由Hg^(2+)诱导罗丹明B的构型从内酰胺螺环(无色无荧光)转变为开环酰胺形态(粉色荧光)。在0~10μmol/L的浓度范围内,探针的荧光强度与Hg^(2+)浓度呈现较好的线性相关性,检出限为0.385μmol/L。此外,通过MALDI-TOF-MS分析实验论证了探针与Hg^(2+)的结合行为和感应机制,充分说明探针中聚乙二醇(PEG)结构在检测Hg^(2+)过程中起着不可或缺的作用。A novel fluorescent sensor derived from the monodisperse 4-Arm-PEG with excellently selective and sensitive for sensing Hg^(2+)in water solution was designed to improve the potential application values of the fluorescent sensor.Fluorescent sensor showed a prominent fluorescence improvement at 572 nm in the presence of Hg^(2+)over other competitive metal cations.The ratiometric fluorescent signal change of the sensor was based on a strict turn-on mechanism,which was adjusted by Hg^(2+)induced configuration transformation of rhodamine from a spirolactam ring to the ring-opening amide.In the range of 0~10μmol/L,the detection limit of Hg^(2+)was 0.385μmol/L.Furthermore,the binding behavior and sensing mechanism of fluorescent sensor to Hg^(2+)were comprehensively illustrated by the MALDI-TOF-MS analysis.It is indicated that PEG structure plays an indispensable role in the detection of Hg^(2+).

关 键 词：荧光探针 汞离子 聚乙二醇 罗丹明B 三氮唑 

分 类 号：O656.3[理学—分析化学]