刺五加多糖对雏鸡脾脏中CD4^(+)和CD8^(+)T淋巴细胞定位分布的影响  

Effects of Acanthopanax senticosus Polysaccharide on Distribution of CD4^(+)and CD8^(+)T Lymphocytes in Chicken Spleen

作　　者：张英楠 徐晶 张桂山 杨树宝 ZHANG Ying-nan;XU Jing;ZHANG Gui-shan;YANG Shu-bao(Jilin Medical University, Jilin 132013, China;College of Life Science, Changchun Sci-Tech University, Changchun, 130600, China;College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China)

机构地区：[1]吉林医药学院,吉林吉林132013 [2]长春科技学院,长春130600 [3]吉林农业大学动物科技学院,长春130118

出　　处：《中国兽药杂志》2021年第9期44-49,共6页Chinese Journal of Veterinary Drug

基　　金：吉林省教育厅“十三五”科学技术项目(JJKH20200355KJ,JJKH20201293 JY);国家自然科学基金资助项目(31372391)。

摘　　要：为了研究刺五加多糖(ASPS)对雏鸡脾脏中CD4^(+)和CD8^(+)T淋巴细胞定位分布的影响,从组织学角度评价ASPS对脾脏的免疫调节作用,试验将1日龄海兰褐公雏饲养至7日龄时选取150只,随机分为3组:空白对照组、ASPS低剂量组(ASPS_(L))和高剂量组(ASPS_(H)),每组50只,所有组每天注射1次,连续注射3 d。免疫后的第7、14、21和28天分别取其脾脏制作冰冻切片,采用免疫组织化学方法检测CD4^(+)和CD8^(+)T淋巴细胞的定位分布。结果显示,与空白对照组比较,免疫注射后21 d和28 d时ASPS_(L)组和ASPS_(H)组CD4^(+)T淋巴细胞的数量均显著增加(P<0.05),而且ASPS能够促进红髓中CD4^(+)T淋巴细胞向动脉周围淋巴鞘迁移,从而使单个动脉周围淋巴鞘面积较对照组明显增加,而ASPS对脾脏中CD8^(+)T淋巴细胞的数量和分布无明显影响。由此可知,ASPS能够通过影响脾脏中CD4^(+)T淋巴细胞的定位分布发挥免疫调节作用,这对于进一步揭示ASPS的免疫调节机制具有重要意义。In order to investigate the effect of Acanthopanax senticosus polysaccharide(ASPS)on the localization and distribution of CD4^(+)and CD8^(+)T lymphocytes in the spleen of chicks,and evaluate the immunomodulatory effect of ASPS on spleen from the perspective of histology,150 Hy-Line brown male chicks at 1-day-old were randomly divided into 3 groups with 50 chicks in each group:blank control group,ASPS low-dose group(ASPS_(L))and ASPS high-dose group(ASPS_(H)).All groups were injected once a day for 3 consecutive days.The distribution of CD4^(+)and CD8^(+)T lymphocytes was detected by immunohistochemistry at 7,14,21 and 28 days after immunization.The results showed that compared with blank control group,the number of CD4+T lymphocytes in ASPS_(L)and ASPS_(H)groups was significantly increased at 21 and 28 days after immunization(P<0.05),and ASPS could promote the migration of CD4^(+)T lymphocytes from the red pulp to the periarterial lymphatic sheath,so that the area of single periarterial lymphatic sheath was significantly increased compared with the control group.However,ASPS had no significant effect on the number and distribution of CD8^(+)T lymphocytes in spleen.The results revealed that ASPS could play an immunomodulatory role by affecting the localization and distribution of CD4^(+)T lymphocytes in the spleen,which is of great significance for further revealing the immunomodulatory mechanism of ASPS.

关 键 词：刺五加多糖 脾脏 CD4^(+)T淋巴细胞 CD8^(+)T淋巴细胞 鸡 定位分布 

分 类 号：S859.79[农业科学—临床兽医学]