高丹草多倍体的诱导与鉴定  

Induction and Identification of Sorghum bicolor×S.sudanense Polyploids

作　　者：王炜[1] 陈琛[1] 葛玉彬[2] 罗俊杰[1] WANG Wei;CHEN Chen;GE Yubin;LUO Junjie(Bio-technology Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China;Crop Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China)

机构地区：[1]甘肃省农业科学院生物技术研究所,兰州730070 [2]甘肃省农业科学院作物研究所,兰州730070

出　　处：《西北农业学报》2021年第9期1394-1401,共8页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：甘肃省农科院生物技术育种专项(2019GAAS07);甘肃省农科院科技支撑计划(2017GAAS44);甘肃省农业生物技术研究与应用开发项目(GNSW-2015-20)。

摘　　要：高丹草(Sorghum bicolor×S.sudanense)是一种优质牧草。为获得高丹草多倍体材料,试验以秋水仙素为诱导剂,以引进的高丹草品种‘大卡’为供试材料,应用浸种法和滴生长点法分别对高丹草种子和幼苗生长点进行了处理,对获得的诱变后代采用表型测定、根尖染色体法和表皮气孔保卫细胞法相结合的方式,通过逐代鉴定筛选,最终在M5代获得了稳定遗传的两份高丹草多倍体材料。本研究可为高丹草多倍体诱导相关研究提供参考;同时创制的两份多倍体材料除可作为种质资源使用外,经进一步培育后有望作为品种在生产上推广应用。Sorghum bicolor×S.sudanense is a kind of elite forage grass.To obtain Sorghum bicolor×S.sudanense polyploids,colchicine was used as chemical mutagen,‘Big Kahuna’as material,the seeds and seedling growth points were treated by the method ofseed-immersion method and drop growth points,respectively.The mutation polyploid progenies of Sorghum bicolor×S.sudanensewere identified by combining the methods of phenotyping,root tip chromosome and epidermal stomatal guard cells.After identification and screening generation by generation,two polyploids obtained in M5 generationweregenetically stable.This study would provide reference for the relevant research of Sorghum bicolor×S.sudanense polyploid induction;and two polyploids created in the study not only can be applied as germplasms,but also are expected to be varieties by further cultivation for promotion and application in grass production.

关 键 词：秋水仙素 染色体 根尖 表皮气孔保卫细胞 倍性鉴定 

分 类 号：S661.2[农业科学—果树学]