黄精属植物DNA分子鉴定技术应用研究进展  

Application of DNA Molecular Identification Technology in Polygonatum Mill.

作　　者：石乃星 文国松[1] 赵明富[2] SHI Nai-xing;WEN Guo-song;ZHAO Ming-fu(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201;Plant Protection College,Yunnan Agricultural University,Kunming 650201)

机构地区：[1]云南农业大学农学与生物技术学院,昆明650201 [2]云南农业大学植物保护学院,昆明650201

出　　处：《植物遗传资源学报》2021年第5期1209-1218,共10页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(81360611)~~。

摘　　要：黄精属植物分布广泛、资源丰富、类群复杂、栽培历史悠久,具有重要的药用、经济、观赏与文化价值。在大健康的时代背景下,黄精作为中国传统中药材,其潜在市场和广泛用途不断受到人们的重视与发掘。随着分子生物学科研究的完善与深入,先后出现了多种基于DNA水平的分子鉴定技术,加速黄精属相关领域的研究并取得了较为丰硕的成果。本文对近年来有关DNA分子鉴定技术(分子标记和DNA条形码)在黄精属植物的应用现状与进展进行了综述,着重综述遗传多样性、种质鉴定、系统发育、分子标记辅助育种等方面的研究进展,并对分子标记技术在黄精属应用中存在的问题及前景进行了讨论,以期在相关领域为今后黄精属的分子标记研究提供参考和对照,并对其资源保护及开发利用提供一定借鉴。The genus Polygonatum Mill.represents a wide geographical distribution,abundant resources,complex taxa and a long cultivation history,and they are considered to be important with the medicinal,economic,ornamental and cultural value.Under the background of the era of the concept of great health,Polygonati Rhizoma as a traditional Chinese herbal medicine,its potential market and wide range of applications are constantly being valued and explored.With the improvement and development of molecular biology research,a variety of molecular identification techniques based on DNA level have emerged,which has accelerated the related research field of Polygonatum and achieved many fruitful results.In this paper,we review the current status and progress of the genus Polygonatum about DNA molecular identification techniques(such as molecular marker and DNA barcode)in recent years,especially focusing on the research of genetic diversity,germplasm identification,phylogeny,etc.The problems and prospects of molecular marker technology are discussed in the application of Polygonatum.We hope to provide references and comparisons for future molecular marker research of Polygonatum in related field and scientific references for efficient protection and utilization of plant resources.

关 键 词：黄精属 分子鉴定 分子标记 DNA条形码 

分 类 号：S567.239[农业科学—中草药栽培]