紫花苜蓿MsFtsH8基因的克隆及功能分析  

Cloning and Functional Analysis of MsFtsH8 Gene from Medicago sativa

作　　者：余倩文 李明娜[2] 龙瑞才[2] 杨青川[2] 康俊梅[2] 郭长虹[1] YU Qian-wen;LI Ming-na;LONG Rui-cai;YANG Qing-chuan;KANG Jun-mei;GUO Chang-hong(Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province/College of Life Science and Technology,Harbin Normal University,Harbin 150000;Beijing Institute of Animal Science,Chinese Academic of Agriculture Science,Beijing 100193)

机构地区：[1]黑龙江省分子细胞遗传与育种重点实验室/哈尔滨师范大学生命科学与技术学院,哈尔滨150000 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《植物遗传资源学报》2021年第5期1442-1451,共10页Journal of Plant Genetic Resources

基　　金：中央级公益性科研院所基本科研业务费专项(2020-YWF-YB-05);中国农业科学院科技创新工程项目(ASTIP-IAS14);哈尔滨师范大学学术创新项目(HSDSSCX2020-09)~~。

摘　　要：盐胁迫是影响植物生长发育的主要非生物胁迫之一。金属蛋白酶FtsH(Filamentation Temperature-Sensitive H)是一种ATP和Zn2+依赖型蛋白酶,广泛存在于真核生物和原核生物中,属于AAA蛋白酶家族(ATPase associated with various cellular activities),在植物叶绿体发育、光合系统蛋白合成和修复、非生物胁迫的应答和抵抗、植物叶片杂色的调控等重要植物生长发育过程中起到了重要的作用。FtsH在拟南芥、油菜、大豆等植物中多有研究,但在紫花苜蓿中的功能还尚未有详细报导。本研究克隆且分析了紫花苜蓿MsFtsH8基因的表达模式,并对该基因进行生物信息学分析,通过在拟南芥中过表达发现,过表达的拟南芥植株在盐胁迫条件下,种子发芽率、根长和鲜重显著提高。研究结果表明,MsFtsH8具有调控耐盐的功能。本研究为苜蓿耐盐分子育种提供了重要候选基因和理论参考。Salt stress is one of the main abiotic stresses affecting plant growth and development.Metalloproteinase FtsH(Filamentation temperature-sensitive H)is a type of ATP and Zn2+dependent protease in the AAA family of proteases(ATPase associated with various cellular activities),and it plays an important role in plant chloroplast development,photosynthetic system protein synthesis and repair,response and resistance to abiotic stress,leaf noise regulation and other important processes.Although FtsH has been extensively studied in several species such as Arabidopsis thaliana(L.)Heynh.,Brassica napus L.,Glycine max(L.)Merr.,its function in Medicago sativa L.has not been reported.In this study,we cloned and analyzed the expression pattern of MsFtsH8 gene in Medicago sativa L.,followed by a bioinformatics analysis of the gene sequence.Over-expression of the gene in Arabidopsis.thaliana(L.)Heynh.significantly increased seed germination rate,root length and fresh weight under salt stress.Collectively,this study provides important candidate genes and theoretical reference for alfalfa molecular breeding regarding to salt tolerance.

关 键 词：紫花苜蓿 金属蛋白酶FtsH 盐胁迫 蛋白水解酶 

分 类 号：S541.9[农业科学—作物学]