瑶药多穗柯提取物对糖尿病大鼠基质金属蛋白酶8、9的影响作用研究  

Effects of Lithocarpus polystachyus on expression of matrix metalloproteinases 8 and 9 in diabetic rats

作　　者：邓聿胤 王丽 吕纪华 刘瑛 覃良 叶银斯 洪芷琴 DENG Yuyin;WANG Li;LYU Jihua;LIU Ying;QIN Liang;YE Yinsi;HONG Zhiqin(Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards,Guangxi Institute of Traditional Medical and Pharmaceutical Sciences,Nanning 530022,China;Liuzhou Red Cross Hospital,Liuzhou 545000,China;Guilin Medical University,Guilin 541004,China)

机构地区：[1]广西壮族自治区中医药研究院/广西中药质量标准研究重点实验室,广西壮族自治区南宁530022 [2]柳州市红十字会医院,广西壮族自治区柳州545000 [3]桂林医学院,广西壮族自治区桂林541004

出　　处：《药学研究》2021年第9期567-570,581,共5页Journal of Pharmaceutical Research

基　　金：广西重点研发计划项目(No.桂科AB17195013)。

摘　　要：目的探讨瑶药多穗柯的降糖作用及作用机制。方法采用高脂饲料+链脲菌素(STZ)制备大鼠糖尿病模型,通过瑶药多穗柯提取物灌胃进行药物干预,检测大鼠体重、血糖、血清胰岛素(INS)、血清糖化血红蛋白(GHbA1c)、血清基质金属蛋白酶8(MMP-8)及基质金属蛋白酶9(MMP-9)水平和胰腺病理等指标,从而观察多穗柯降糖作用。结果与模型组比较,多穗柯提取物7 g生药/kg剂量组大鼠体重增加,血糖降低,血清糖化血红蛋白及基质金属蛋白酶9水平降低,血清胰岛素水平升高,胰腺病理病变明显改善。结论瑶药多穗柯7 g生药/kg剂量具有显著降糖作用,其机制可能与改善胰岛功能,促进胰岛素分泌,调控糖化血红蛋白与基质金属蛋白酶9水平相关。Objective To study the hypoglycemic effect of Lithocarpus polystachyus and its mechanism.Methods Diabetic rat model induced by high-fat diet feeding and Streptozotocin Injection,Through giving the Lithocarpus polystachyus extract to observe the effect of polycotyledon extract on body weight,blood glucose,serum insulin,serum glycated hemoglobin,serum matrix metalloproteinase 8,serum matrix metalloproteinase 9 and pathological changes of pancreas in rats.Results Compared with the model group,Lithocarpus polystachyus extract which dosage was 7 g crude drug/kg could increase the weight of diabetic rats,decrease the levels of fasting blood glucose,serum GHbA1c and serum MMP-9,increase the levels of serum INS,and improve the changes of pancreatic tissue.Conclusion Lithocarpus polystachyus has significant hypoglycemic effect,which may be related to the improvement of islet function,insulin secretion,regulation of glycated hemoglobin and MMP-9 level.

关 键 词：糖尿病大鼠 多穗柯 胰岛素 糖化血红蛋白 基质金属蛋白酶 

分 类 号：R285.5[医药卫生—中药学]