HPLC法测定消栓通络片中3种三七成分的含量  

Determination of 3 ingredients of Panax notoginseng in Xiaoshuan Tongluo Tablets by HPLC

作　　者：张桂英[1] 许崇德[1] 刘建芳[1] 牛家丰 ZHANG Guiying;XU Chongde;LIU Jianfang;NIU Jiafeng(People'Hospital of Rizhao,Rizhao 276800,China;Zaozhuang Institute for Food and Drug Control,Zaozhuang 277299,China)

机构地区：[1]日照市人民医院,山东日照276800 [2]枣庄市食品药品检验检测中心,山东枣庄277299

出　　处：《药学研究》2021年第9期578-581,共4页Journal of Pharmaceutical Research

摘　　要：目的建立一种利用高效液相技术同时测定消栓通络片中3种三七成分含量的方法。方法采用安捷伦1200型高效液相色谱仪,InerSustain C 18色谱柱,检测波长为203 nm,乙腈-水为流动相,梯度洗脱,流速为1.0 mL·min^(-1)。结果在此分离条件下,三七中的人参皂苷Rb_(1)、Rg_(1)与三七皂苷R_(1)的色谱峰能够很好分离,分别在0.025~0.51、0.026~0.52、0.025~0.51 mg·mL^(-1)的范围内与峰面积呈良好线性关系,加样回收率分别为98.12%、99.68%、100.51%,RSD分别为2.87%、2.01%、2.16%。不同厂家生产的消栓通络片中3种三七成分的含量差异不明显,未检出非法添加三七茎叶提取物的样品。结论该方法操作简便、专属性强、结果准确,可为消栓通络片质量检测方法的制定提供参考。Objective To establish a reversed-phase HPLC method for the content determination of ginsenoside Rb_(1),Rg_(1) and notoginsenoside R_(1) in Xiaoshuan Tongluo Tablets.Methods The sample was separated on Agilent 1200 Series HPLC,employed with an InerSustain C 18 column,and aqueous acetonitrile was used as mobile gradient elution.The flow rate was 1.0 mL·min^(-1),and the detection wave length was set at 203 nm.Results Ginsenoside Rb_(1),Rg_(1) and notoginsenoside R_(1) were separated well from other constituents by this HPLC method.The calibration curves were linear in the ranges of 0.025~0.51 mg·mL^(-1) for ginsenoside Rb 1(r=0.9997),0.026~0.52 mg·mL^(-1) for ginsenoside Rg_(1)(r=0.9997)and 0.025~0.51 mg·mL^(-1) for notoginsenoside R 1(r=0.9996).Their average recoveries were 98.12%,99.68% and 100.51% with RSD values at 2.87%,2.01% and 2.16%(n=6),respectively.Contents of ginsenoside Rb_(1),Rg_(1) and notoginsenoside R_(1) in Xiaoshuan Tongluo Tablets from different manufacturers revealed no obvious difference,and no sample was detected to add with the extract of Panax notoginseng stems and leaves.Conclusion This established method was sensitive,accurate and can be used for the quality control of ginsenoside Rb_(1),Rg_(1) and notoginsenoside R_(1) in Xiaoshuan Tongluo Tablets.

关 键 词：消栓通络片 三七 人参皂苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]